New HMM profiles were created based on UniLectin3D updated lectin classes and a large proportion of existing profiles were revised. On this basis, LectomeXplore was updated with new protein content from UniProt (release of October 2025) and NCBI nr (release of September 2025), and with new domain content from InterPro (release of October 2025), thereby increasing the number of predictions by 42%.

UniLectin3D was enriched with 170 new structures requiring for some, the definition of new lectin classes, which brought the number from previous 109 up to current 117. Three new commercial lectins were added to BiotechLec. Human Lectome references and ligands were updated based on the latest publications.

Completed PathoLec module, fully integrated in UniLectin. PathoLec contains curated lectins from known pathogenic agents based on information extracted from SugarBindDB. In complement to glycan-binding and species details usually found in UniLectin, this module includes descriptions of the host organisms, associated diseases and affected host tissues. Each considered SugarBindDB entry was manually verified, curated and updated, before integration into PathoLec.

New ligand search tool implemented in UniLectin3D. Ligand input is either in IUPAC or GlycoCT format, as well as GlyTouCan ID or glycan composition. Each ligand search hit links to lectin entries where a ligand hit-lectin interaction is reported.

(1) Security: enhanced protection against injections and database attacks, in particular for the API. (2) Expanded documentation and refined layout of the API page: (a) introduction of new API endpoints for programmatic access to BiotechLec, HumanLectome, PropLec, MycoLec and TrefLec data and (b) expansion of UniLectin3D and LectomeXplore endpoints, granting open access to additional data types. More information can be obtained on unilectin.unige.ch/api.

A new tool to compare the distribution of the lectin classes and folds across species has been added to LectomeXplore on the unilectin.unige.ch/predict/class-phylogeny page. Comparison is achieved via an interactive heatmap across a maximum of ten user-selected phylogenetic groups, at user-specified score threshold.

(1) Redesigned homepage: the homepage layout was reshaped for an improved user experience. (2) Introducing a new UniLectin logo: UniLectin is part of the Glyco@Expasy collection and the new design shares graphical features with other resources of the collection. (3) Enhanced citation display: References for citation are displayed in a pop-up window instead of a list in the footer. (4) More flexible UniLectin3D origin search: broader coverage of organisms and partial matches accepted.

(1) Norovirus known to cause gastroenteritis, bind human blood group oligosaccharides through their capsid proteins. This lectin class was updated in UniLectin3D to include 178 manually curated structures, correctly assigned to their genotype. The addition of 60 new structures is supported by the GlycoNoVI EU-MSCA project. (2) The consistency of ligand curation in UniLectin3D was revised in order to better capture the relationship between lectins and ligands. By organising the latter as separate entities, ligands can also be considered as sub-structures of larger glycan structures.

The loading of the different pages of UniLectin3D and LectomeXplore has been drastically shortened and is now performed in the background for a smoother user experience. To further optimize the loading time of recurrent users, caching has been implemented for the heaviest functionalities. Finally, the user can now select a score threshold when browsing the predicted lectins by fold and origin in LectomeXplore.

The present release includes the update of the server, website libraries, frameworks and tools. It also fixes several vulnerabilities to injections and better secures the search tools.