NCBI Summary
Sialic acid-binding immunoglobulin (Ig)-like lectins, or SIGLECs (e.g., CD33 (MIM 159590)), are a family of type 1 transmembrane proteins each having a unique expression pattern, mostly in hemopoietic cells. SIGLEC8 is a member of the CD33-like subgroup of SIGLECs, which are localized to 19q13.3-q13.4 and have 2 conserved cytoplasmic tyrosine-based motifs: an immunoreceptor tyrosine-based inhibitory motif, or ITIM (see MIM 604964), and a motif homologous to one identified in signaling lymphocyte activation molecule (SLAM; MIM 603492) that mediates an association with SLAM-associated protein (SAP; MIM 300490) (summarized by Foussias et al., 2000 [PubMed 11095983]).[supplied by OMIM, May 2010].
Protein
Protein (NP_055257)
SIGLEC-8
Sialic acid-binding Ig-like lectin 8 (Siglec-8) (Sialoadhesin family member 2) (SAF-2)
SIGLEC8
sialic acid binding Ig like lectin 8
CD329
Curated
I-type lectin
I-Type Lectins - CD33-related Siglecs
b-sandwich / Ig-like
NeuAc / Neu5Ac(a2-6)(6S)Gal(b1-4)GlcNAc
NP_055257
27181
Q9NYZ4
ENSP00000321077.2
Q9NYZ4
0.541
10.1146/annurev-immunol-102419-035900
neXtProt, PFAM, InterPro, CFG data
Protein sequence and protein families (fasta) (499 amino acids) Download
MLLLLLLLPLLWGTKGMEGDRQYGDGYLLQVQELVTVQEGLCVHVPCSFSYPQDGWTDSDPVHGYWFRAGDRPYQDAPVATNNPDREVQAETQGRFQLLGDIWSNDCSLSIRDARKRDKGSYFFRLERGSMKWSYKSQLNYKTKQLSVFVTALTHRPDILILGTLESGHSRNLTCSVPWACKQGTPPMISWIGASVSSPGPTTARSSVLTLTPKPQDHGTSLTCQVTLPGTGVTTTSTVRLDVSYPPWNLTMTVFQGDATASTALGNGSSLSVLEGQSLRLVCAVNSNPPARLSWTRGSLTLCPSRSSNPGLLELPRVHVRDEGEFTCRAQNAQGSQHISLSLSLQNEGTGTSRPVSQVTLAAVGGAGATALAFLSFCIIFIIVRSCRKKSARPAAGVGDTGMEDAKAIRGSASQGPLTESWKDGNPLKKPPPAVAPSSGEEGELHYATLSFHKVKPQDPQGQEATDSEYSEIKIHKRETAETQACLRNHNPSSKEVRG
Mol* PDB structure viewerUniLectin3D
Structural models
AlphaFold v2: AF-Q9NYZ4-F1Download
  Model Confidence:
  •    Very high (pLDDT > 90)
  •    Confident (90 > pLDDT > 70)
  •    Low (70 > pLDDT > 50)
  •    Very low (pLDDT < 50)

  AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

AlphaFold_v2 structural model assessment through SWISS-MODEL tools.

Ligand
Glycan ligands from structural data
aNeuAc23bGal(6SO3)14(aFuc13)GlcNAc
NeuAc(a2-3)Gal(b1-4)[Fuc(a1-3)]GlcNAc
2N7B
2N7B
GlyConnect Structures
Expression
Human Protein Atlas
GeneCards
mRNA expression in normal human tissues for SIGLEC8 Gene
ProteomicsDB
References
NCBI References (10 PubMed Identifiers)
  • First Evidence for a Role of Siglec-8 in Breast Cancer. [33670444]
  • Promoter region single nucleotide polymorphism of siglec-8 gene associates with susceptibility to allergic asthma. [32077788]
  • Sialic acid-binding immunoglobulin-like lectin (Siglec) 8 in patients with eosinophilic disorders: Receptor expression and targeting using chimeric antibodies. [30543818]
  • Sialic acid-binding immunoglobulin-like lectin 8 (Siglec-8) is an activating receptor mediating beta2-integrin-dependent function in human eosinophils. [28888781]
  • Leveraging Siglec-8 endocytic mechanisms to kill human eosinophils and malignant mast cells. [28734845]
  • Ligation of Siglec-8: a selective mechanism for induction of human eosinophil apoptosis. [12609831]
  • Identification, characterization and leucocyte expression of Siglec-10, a novel human sialic acid-binding receptor. [11284738]
  • Molecular characterization of a Siglec8 variant containing cytoplasmic tyrosine-based motifs, and mapping of the Siglec8 gene. [11095983]
  • Identification of SAF-2, a novel siglec expressed on eosinophils, mast cells, and basophils. [10856141]
  • Siglec-8. A novel eosinophil-specific member of the immunoglobulin superfamily. [10625619]
UniProt Main References (3 PubMed Identifiers)
  • The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). [15489334]
  • Structural basis for sulfation-dependent self-glycan recognition by the human immune-inhibitory receptor Siglec-8. [27357658]
  • Exome sequencing identifies frequent mutation of the SWI/SNF complex gene PBRM1 in renal carcinoma. [21248752]
All isoforms of this gene containing a lectin domain
NP_055257.2, XP_011525036.1, NP_001350477.1
RNA
RNA (Transcript ID: NM_014442.3)
NM_014442.3
sialic acid binding Ig like lectin 8, transcript variant 1
All isoforms of SIGLEC8 containing a lectin domain
ENST00000321424.7
68..1567 (or 51452379.. 51458387)
51450997.. 51458454
NCBI Matured mRNA sequence 2949 NTPDownload 
m7G-5')ppp(5'-AGUUUCUGAGAGAAGAACCCUGAGGAACAGACGUUCCCUGGCGGCCCUGGCGCCUUCAAACCCAGACAUGCUGCUGCUGCUGCUGCUGCUGCCCCUGCUCUGGGGGACAAAGGGGAUGGAGGGAGACAGACAAUAUGGGGAUGGUUACUUGCUGCAAGUGCAGGAGCUGGUGACGGUGCAGGAGGGCCUGUGUGUCCAUGUGCCCUGCUCCUUCUCCUACCCCCAGGAUGGCUGGACUGACUCUGACCCAGUUCAUGGCUACUGGUUCCGGGCAGGAGACAGACCAUACCAAGACGCUCCAGUGGCCACAAACAACCCAGACAGAGAAGUGCAGGCAGAGACCCAGGGCCGAUUCCAACUCCUUGGGGACAUUUGGAGCAACGACUGCUCCCUGAGCAUCAGAGACGCCAGGAAGAGGGAUAAGGGGUCAUAUUUCUUUCGGCUAGAGAGAGGAAGCAUGAAAUGGAGUUACAAAUCACAGUUGAAUUACAAAACUAAGCAGCUGUCUGUGUUUGUGACAGCCCUGACCCAUAGGCCUGACAUCCUCAUCCUAGGGACCCUAGAGUCUGGCCACUCCAGGAACCUGACCUGCUCUGUGCCCUGGGCCUGUAAGCAGGGGACACCCCCCAUGAUCUCCUGGAUUGGGGCCUCCGUGUCCUCCCCGGGCCCCACUACUGCCCGCUCCUCAGUGCUCACCCUUACCCCAAAGCCCCAGGACCACGGCACCAGCCUCACCUGUCAGGUGACCUUGCCUGGGACAGGUGUGACCACGACCAGUACCGUCCGCCUCGAUGUGUCCUACCCUCCUUGGAACUUGACCAUGACUGUCUUCCAAGGAGAUGCCACAGCAUCCACAGCCCUGGGAAAUGGCUCAUCUCUUUCAGUCCUUGAGGGCCAGUCUCUGCGCCUGGUCUGUGCUGUCAACAGCAAUCCCCCUGCCAGGCUGAGCUGGACCCGGGGGAGCCUGACCCUGUGCCCCUCACGGUCCUCAAACCCUGGGCUGCUGGAGCUGCCUCGAGUGCACGUGAGGGAUGAAGGGGAAUUCACCUGCCGAGCUCAGAACGCUCAGGGCUCCCAGCACAUUUCCCUGAGCCUCUCCCUGCAGAAUGAGGGCACAGGCACCUCAAGACCUGUAUCACAAGUGACACUGGCAGCAGUCGGGGGAGCUGGAGCCACAGCCCUGGCCUUCCUGUCCUUCUGCAUCAUCUUCAUCAUAGUGAGGUCCUGCAGGAAGAAAUCGGCAAGGCCAGCAGCGGGCGUGGGGGAUACAGGCAUGGAAGAUGCAAAGGCCAUCAGGGGCUCGGCCUCUCAGGGACCCCUGACUGAAUCCUGGAAAGAUGGCAACCCCCUGAAGAAGCCUCCCCCAGCUGUUGCCCCCUCGUCAGGGGAGGAAGGAGAGCUCCAUUAUGCAACCCUCAGCUUCCAUAAAGUGAAGCCUCAGGACCCGCAGGGACAGGAGGCCACUGACAGUGAAUACUCGGAGAUCAAGAUCCACAAGCGAGAAACUGCAGAGACUCAGGCCUGUUUGAGGAAUCACAACCCCUCCAGCAAAGAAGUCAGAGGCUGAUUCUCAUAGAACAAGAACCCUCUAGAGCCCCAUGCUAUGCAGUAGGUCACCAGGGCUCCCUCCUCCUGUCUAACCAAAACUUGGACCAAUGUCUCCCCUUUCCCCGGCUACCAGGGACCCAUCCCUGCCUCUAGCUUCUACUACCCACCAUUCUCCUCUCGACCUCUCUGAGGUUGACUAUUUUAGAUUCCACAUAGAGAUGAGGUCAUGUGGUACUUGCCUCUCUGUGUGUGGCUCAUUUUACACAAAAAAAUAUCCCCUAGGUUCAUCCAUGUUCUCUCAAAUGACAGAAUCAAGCACUGAAUAUUUUUUUUUCUUUGAGAGAUGGAGUUUCGCUCUGUUGCCCAGGCUGGAGUGCAGUGGUUCAAUCUCUGCUCACUGCAACCUCCACCUCCUGGGUUCAAACGAUUCUCCUGCCUCAGCUUCCCAAGUAGCUGGUACUACAGGCGUGUGUCACCACGCCCAGCUAAUUUUUGUAUUUUUUAGUAGAGACGGGGUUUCACUAUAAGUGGGCCAGGCUAGUCUCAAACUCCUGACCUCAAGUGAUCUGCCUGCCUUGGCCUCCCAAAGUGCUGGGAUUUCAGGCAUGAGCCACCGCACCCAGCUUGCAUUGAAUAUUUUCAAGGAGCUAAAAGAAGAUUUUAAAUGGUCUCACAAAAACAGAUAAAUAUUUGCACAGAUGGGUGUGCUAAUCAUUGUGCCUUGAUGGUUCCACGAUGUAUCCGGGUGUGGAAAUCUCACUGGGUCUCUCUCAAGGCCACUCGGCUACUCAGGACAGGGCUGGAAUUUAAAGCCUGUCCGAUUCUGAGGUCUCUUCUCUCAUCUAGCACUGAGUCAAGCAAUCAGCAGGCUGGGCACCCCUUAGCCAUAAGUUUUCAGGAAAUAAAUUCCUUGAGGGCAUUGACUUUUACAAAAGAGGGAGCAGCAAUGGCCUAGAGUCUCAGGAACAAGACAGGUGCACUGAGGAGAUGAAGGCCGGGACCCCCUGCCCAACCUGUAUGGCGGGUCUGUACUUAUUUUGUUUACCCCCAAUUUAAAACGUUUUUUUUUAUUGCAGGUUGUUUGUUUGAUAUGGUUUGGCUGUGUCCCCACCCAAAUCUUAUCUAGAAUUGUAAUCAGAAUUAUAAUCCCCAUGUGUUGGGGGAGGGACCUGGUGGGAGGUGAUAGGAUCAUGGGGGUGGUUCCCCCAUGCUGUUCUGAUAGUGAGUGAGUUAUCACGAGAUCUGAUGGUUUUGUAAGUGGUGGUUUCCCCUGCUCUUCUCUCUUGCCUGCCACCAUGUAAGAUGUGCCUGGUUCCCCUUCCGCCAUGAUUGUAAGUUUCCUGAGGCCUCCCCCGCCAUGUGGAACUGUGAGUCAAUUACACCUCUUUCAUUUAUAAAUUA-3'- Poly-A tail
  • Coding region
NCBI Matured mRNA sequence viewer
;
More about the NCBI sViewer.
DNA
DNA (Gene ID: 27181)
SIGLEC8
sialic acid binding Ig like lectin 8
NC_000019.10
19  51452379.. 51458387
51452379.. 51458387
strand -
CCDS33086.1
MGI:1932475
ClinVar, dbVar
ENSG00000105366
SIGLEC-8, SAF2, SIGLEC8L, MGC59785
uc002pwt.3
INSDC: AF195092
10877
605639
OTTHUMG00000182776
ENA, DDBJ, Monarch
NCBI CDS Gene viewer
More about the NCBI sViewer.
NCBI CDS gene sequence (1500 bp)
5'-ATGCTGCTGCTGCTGCTGCTGCTGCCCCTGCTCTGGGGGACAAAGGGGATGGAGGGAGACAGACAATATGGGGATGGTTACTTGCTGCAAGTGCAGGAGCTGGTGACGGTGCAGGAGGGCCTGTGTGTCCATGTGCCCTGCTCCTTCTCCTACCCCCAGGATGGCTGGACTGACTCTGACCCAGTTCATGGCTACTGGTTCCGGGCAGGAGACAGACCATACCAAGACGCTCCAGTGGCCACAAACAACCCAGACAGAGAAGTGCAGGCAGAGACCCAGGGCCGATTCCAACTCCTTGGGGACATTTGGAGCAACGACTGCTCCCTGAGCATCAGAGACGCCAGGAAGAGGGATAAGGGGTCATATTTCTTTCGGCTAGAGAGAGGAAGCATGAAATGGAGTTACAAATCACAGTTGAATTACAAAACTAAGCAGCTGTCTGTGTTTGTGACAGCCCTGACCCATAGGCCTGACATCCTCATCCTAGGGACCCTAGAGTCTGGCCACTCCAGGAACCTGACCTGCTCTGTGCCCTGGGCCTGTAAGCAGGGGACACCCCCCATGATCTCCTGGATTGGGGCCTCCGTGTCCTCCCCGGGCCCCACTACTGCCCGCTCCTCAGTGCTCACCCTTACCCCAAAGCCCCAGGACCACGGCACCAGCCTCACCTGTCAGGTGACCTTGCCTGGGACAGGTGTGACCACGACCAGTACCGTCCGCCTCGATGTGTCCTACCCTCCTTGGAACTTGACCATGACTGTCTTCCAAGGAGATGCCACAGCATCCACAGCCCTGGGAAATGGCTCATCTCTTTCAGTCCTTGAGGGCCAGTCTCTGCGCCTGGTCTGTGCTGTCAACAGCAATCCCCCTGCCAGGCTGAGCTGGACCCGGGGGAGCCTGACCCTGTGCCCCTCACGGTCCTCAAACCCTGGGCTGCTGGAGCTGCCTCGAGTGCACGTGAGGGATGAAGGGGAATTCACCTGCCGAGCTCAGAACGCTCAGGGCTCCCAGCACATTTCCCTGAGCCTCTCCCTGCAGAATGAGGGCACAGGCACCTCAAGACCTGTATCACAAGTGACACTGGCAGCAGTCGGGGGAGCTGGAGCCACAGCCCTGGCCTTCCTGTCCTTCTGCATCATCTTCATCATAGTGAGGTCCTGCAGGAAGAAATCGGCAAGGCCAGCAGCGGGCGTGGGGGATACAGGCATGGAAGATGCAAAGGCCATCAGGGGCTCGGCCTCTCAGGGACCCCTGACTGAATCCTGGAAAGATGGCAACCCCCTGAAGAAGCCTCCCCCAGCTGTTGCCCCCTCGTCAGGGGAGGAAGGAGAGCTCCATTATGCAACCCTCAGCTTCCATAAAGTGAAGCCTCAGGACCCGCAGGGACAGGAGGCCACTGACAGTGAATACTCGGAGATCAAGATCCACAAGCGAGAAACTGCAGAGACTCAGGCCTGTTTGAGGAATCACAACCCCTCCAGCAAAGAAGTCAGAGGCTGA-3'
NCBI CDS gene sequence with introns (location: 51452379.. 51458387) (6009 bp)Download
NCBI Gene Viewer (CDS + introns)
NCBI CDS gene sequence with introns, 5'UTR and 3'UTR (location: 51450997.. 51458454) (7458 bp)Download
NCBI Gene Viewer (CDS + introns + Untranslated regions)
NCBI gene sequence (location: [51450997.. 51458454 + 1000]) (8458 bp)Download
NCBI Gene Viewer (CDS + introns + Untranslated regions + 1000 dNTP to potentially reach the promotor)
How to cite: Schnider B., M'Rad Y., el Ahmadie J., de Brevern AG., Imberty A., Lisacek F., HumanLectome, an update of UniLectin for the annotation and prediction of human lectins, Nucleic Acids Reasearch doi.org/10.1093/nar/gkad905