HumanLectome - Human Lectin Entry

NCBI Summary

This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. The encoded type II transmembrane protein is a downstream target of CCAAT/enhancer binding protein (C/EBP), beta (CEBPB) and may play a role in inflammation. Alternative splice variants have been described but their full-length sequence has not been determined. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region. [provided by RefSeq, Jul 2008].

Protein

Protein (NP_055173)

Protein name

Mincle

Synonym(s)

C-type lectin domain family 4 member E (C-type lectin superfamily member 9) (Macrophage-inducible C-type lectin) (MINCLE)

HGNC gene name

CLEC4E

HGNC name

C-type lectin domain family 4 member E

Protein_CD_name

Undefined

Lectin confidence

Curated

UniLectin class

C-type lectin

C-type classification

CLEC4E

Known lectin group

C-type - Type II transmembrane receptors

Lectin fold

a/b mixed / C-type lectin-like

Glycan specificity

Trehalose / Glycolipid

RefSeq accession

Gene ID

UniProt accession

Ensembl protein ID

UniRef cluster ID

LectomeXplore score

0.432

Reference

10.1073/pnas.1312649110

Other links

PFAM, InterPro, CFG data

Protein sequence and protein families (fasta) (219 amino acids) Download

MNSSKSSETQCTERGCFSSQMFLWTVAGIPILFLSACFITRCVVTFRIFQTCDEKKFQLPENFTELSCYNYGSGSVKNCCPLNWEYFQSSCYFFSTDTISWALSLKNCSAMGAHLVVINSQEEQEFLSYKKPKMREFFIGLSDQVVEGQWQWVDGTPLTKSLSFWDVGEPNNIATLEDCATMRDSSNPRQNWNDVTCFLNYFRICEMVGINPLNKGKSL

Mol* PDB structure viewerUniLectin3D

Select a PDB entry:

Structural models

AlphaFold v2: AF-Q9ULY5-F1 Download

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Oligomerization and Known Interactions

Monomer and homodimer (PubMed:18509109). Interacts with signaling adapter Fc receptor gamma chain/FCER1G to form a functional complex; the interaction is direct (By similarity). Alternatively, acts as a bridge for interaction between CLEC4D and FCER1G. A heterodimer of CLEC4E and CLEC4D associates with FCER1G to form a functional complex (By similarity). Interacts with SAP130 nuclear protein that is released from necrotic cells; the interaction is direct (By similarity). Source: UniProt

Annotation

Ligand

Glycan ligands from structural data

No crystal structures of complexes with glycan ligand.

Expression

Human Protein Atlas

GeneCards

Functionality temporarily unavailable.

References

NCBI References (10 PubMed Identifiers)

A case-control study on correlation between the single nucleotide polymorphism of CLEC4E and the susceptibility to tuberculosis among Han people in Western China. [34376176]

A reference map of the human binary protein interactome. [32296183]

Molecular mechanism of obesity-induced adipose tissue inflammation. the role of Mincle in adipose tissue fibrosis and ectopic lipid accumulation. [31852849]

Immune Recognition of Pathogen-Derived Glycolipids Through Mincle. [32152942]

Association between CLEC4E gene polymorphism of mincle and pulmonary tuberculosis infection in a northern Chinese population. [31075410]

Crosstalk between components of the innate immune system: promoting anti-microbial defenses and avoiding immunopathologies. [19120481]

Human and mouse macrophage-inducible C-type lectin (Mincle) bind Candida albicans. [18509109]

Evolutionary analysis reveals collective properties and specificity in the C-type lectin and lectin-like domain superfamily. [12945048]

A novel LPS-inducible C-type lectin is a transcriptional target of NF-IL6 in macrophages. [10528209]

C-type lectin-like domains. [10508765]

UniProt Main References (8 PubMed Identifiers)

The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment. [12975309]

Complete sequencing and characterization of 21,243 full-length human cDNAs. [14702039]

The finished DNA sequence of human chromosome 12. [16541075]

The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). [15489334]

The macrophage-inducible C-type lectin, mincle, is an essential component of the innate immune response to Candida albicans. [18490740]

Mincle is an ITAM-coupled activating receptor that senses damaged cells. [18776906]

C-type lectin MCL is an FcRgamma-coupled receptor that mediates the adjuvanticity of mycobacterial cord factor. [23602766]

Structural analysis for glycolipid recognition by the C-type lectins Mincle and MCL. [24101491]

All isoforms of this gene containing a lectin domain

XP_011518916.1, NP_055173.1, XP_011518917.1

RNA

RNA (Transcript ID: NM_014358.4)

RNA RefSeq ID

NM_014358.4

RNA name

C-type lectin domain family 4 member E

Isoforms

All isoforms of CLEC4E containing a lectin domain

RNA Ensembl ID

ENST00000299663.8

Coding region

109..768 (or 8534638.. 8540797)

mRNA location

8533305.. 8540905

NCBI Matured mRNA sequence 2101 NTP Download

m⁷G-5')ppp(5'-AUUCAUUCUCCCUGAAUCUUACCAACAAAACACUCCUGAGGAGAAAGAAAGAGAGGGAGGGAGAGAAAAAGAGAGAGAGAGAAACAAAAAACCAAAGAGAGAGAAAAAAUGAAUUCAUCUAAAUCAUCUGAAACACAAUGCACAGAGAGAGGAUGCUUCUCUUCCCAAAUGUUCUUAUGGACUGUUGCUGGGAUCCCCAUCCUAUUUCUCAGUGCCUGUUUCAUCACCAGAUGUGUUGUGACAUUUCGCAUCUUUCAAACCUGUGAUGAGAAAAAGUUUCAGCUACCUGAGAAUUUCACAGAGCUCUCCUGCUACAAUUAUGGAUCAGGUUCAGUCAAGAAUUGUUGUCCAUUGAACUGGGAAUAUUUUCAAUCCAGCUGCUACUUCUUUUCUACUGACACCAUUUCCUGGGCGUUAAGUUUAAAGAACUGCUCAGCCAUGGGGGCUCACCUGGUGGUUAUCAACUCACAGGAGGAGCAGGAAUUCCUUUCCUACAAGAAACCUAAAAUGAGAGAGUUUUUUAUUGGACUGUCAGACCAGGUUGUCGAGGGUCAGUGGCAAUGGGUGGACGGCACACCUUUGACAAAGUCUCUGAGCUUCUGGGAUGUAGGGGAGCCCAACAACAUAGCUACCCUGGAGGACUGUGCCACCAUGAGAGACUCUUCAAACCCAAGGCAAAAUUGGAAUGAUGUAACCUGUUUCCUCAAUUAUUUUCGGAUUUGUGAAAUGGUAGGAAUAAAUCCUUUGAACAAAGGAAAAUCUCUUUAAGAACAGAAGGCACAACUCAAAUGUGUAAAGAAGGAAGAGCAAGAACAUGGCCACACCCACCGCCCCACACGAGAAAUUUGUGCGCUGAACUUCAAAGGACUUCAUAAGUAUUUGUUACUCUGAUAUAAAUAAAAAUAAGUAGUUUUAAAUGUUAUAAUUCAUGUUACUGGCUGAAGUGCAUUUUCUCUCUACGUUAGUCUCAGGUCCUCUUCCCAGAAUUUACAAAGCAAUUCACUACCUUUUGCUACAUUUGCCUCAUUUUUUAGUGUUCGUAUGAAAGUACAGGGACACGGAGCCAAGACAGAGUCUAGCAAAGAAGGGGAUUUUGGAAGGUGCCUUCCAAAAAUCUCCUGAAUCCGGGCUCUGUAGCAGGUCCUCUUCUUUCUAGCUUCUGACAAGUCUGUCUUCUCUUCUUGGUUUCAUACCGUUCUUAUCUCCUGCCCAAGCAUAUAUCGUCUCUUUACUCCCCUGUAUAAUGAGUAAGAAGCUUCUUCAAGUCAUGAAACUUAUUCCUGCUCAGAAUACCGGUGUGGCCUUUCUGGCUACAGGCCUCCACUGCACCUUCUUAGGGAAGGGCAUGCCAGCCAUCAGCUCCAAACAGGCUGUAACCAAGUCCACCCAUCCCUGGGGCUUCCUUUGCUCUGCCUUAUUUUCAAUUGACUGAAUGGAUCUCACCAGAUUUUGUAUCUAUUGCUCAGCUAGGACCCGAGUCCAAUAGUCAAUUUAUUCUAAGCGAACAUUCAUCUCCACACUUUCCUGUCUCAAGCCCAUCCAUUAUUUCUUAACUUUUAUUUUAGCUUUCGGGGGUACAUGUUAAAGGCUUUUUAUAUAGGUAAACUCAUGUCGUGGAGGUUUGUUGUACAGAUUAUUUCAUCACCCAGGUAUUAAGCCCAGUGCCUAAUAUUGUUUUUUUCGGCUCCUCUCCCUCCUCCUACCUUCCGCCCUCAAGUAGACUCCAGUGUCUGUUAUUCCCUUCUUUGUGUUUAUGAAUUCUCAUCAUUUAGCUCCCACUUAUAAGUGAGGACAUGCAGUAUUUGGUUUUCUGUUCCCAUGUUUGCUAAGGAUAAUGGUCUCCAGUUCUACCGAUGUUCCCACAAAAGACAUAAUCUUCUUUUUUAAGGCUGCUUAGUAUUCCAUGGUAUCUAUGUAUCACAUUUUCUCUAUCCAAUCUAUUGUUGACUCACAUUUAGAUUGAUUCCAUGUUUUUGCUAUUGUGAAUAGUGCUGCAAUGAACAUUCGUGUGCAUGUGUCUUUAUGGUAGAAAGAUUUAUAUUUCUCUGAGUAUGUAUCCAGUAAUAGCCCAUUCAUUUAUUGCAUAAAAUUCUACCAAUAC-3'- Poly-A tail

Coding region

mRNA-related data

DNA

DNA (Gene ID: 26253)

HGNC Gene symbol

CLEC4E

HGNC Gene name

C-type lectin domain family 4 member E

Chr. RefSeq ID

NC_000012.12

Chromosome N°

12 8534638.. 8540797

CDS location

8534638.. 8540797

DNA strand

strand -

CCDS ID

CCDS8594.1

Mouse ortholog gene

MGI:1861232

Gene Variant

ClinVar, dbVar

DNA Ensembl ID

ENSG00000166523

HGNC other symbol

MINCLE

UCSC ID

uc001quo.2

GeneBank

INSDC: AB024718

GeneCards

14555

MIM

609962

VEGA ID

OTTHUMG00000168675

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