HumanLectome - Human Lectin Entry

NCBI Summary

Calreticulin is a highly conserved chaperone protein which resides primarily in the endoplasmic reticulum, and is involved in a variety of cellular processes, among them, cell adhesion. Additionally, it functions in protein folding quality control and calcium homeostasis. Calreticulin is also found in the nucleus, suggesting that it may have a role in transcription regulation. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin. Recurrent mutations in calreticulin have been linked to various neoplasms, including the myeloproliferative type.[provided by RefSeq, May 2020].

Protein name

Calreticulin

Synonym(s)

Calreticulin (CRP55) (Calregulin) (Endoplasmic reticulum resident protein 60) (ERp60) (HACBP) (grp60)

HGNC gene name

CALR

HGNC name

calreticulin

Protein_CD_name

Undefined

Lectin confidence

Curated

UniLectin class

calnexin-calreticulin like

Known lectin group

L-Type Lectins

Lectin fold

b-sandwich / ConA-like

Glycan specificity

Glc(a1-3)Man(a1-2)Man(a1-2)Man / glucosylated N-glycan

RefSeq accession

Gene ID

UniProt accession

Ensembl protein ID

UniRef cluster ID

LectomeXplore score

0.626

Reference

10.1021/bi972465g

Other links

neXtProt, PFAM, InterPro

Protein sequence and protein families (fasta) (417 amino acids) Download

MLLSVPLLLGLLGLAVAEPAVYFKEQFLDGDGWTSRWIESKHKSDFGKFVLSSGKFYGDEEKDKGLQTSQDARFYALSASFEPFSNKGQTLVVQFTVKHEQNIDCGGGYVKLFPNSLDQTDMHGDSEYNIMFGPDICGPGTKKVHVIFNYKGKNVLINKDIRCKDDEFTHLYTLIVRPDNTYEVKIDNSQVESGSLEDDWDFLPPKKIKDPDASKPEDWDERAKIDDPTDSKPEDWDKPEHIPDPDAKKPEDWDEEMDGEWEPPVIQNPEYKGEWKPRQIDNPDYKGTWIHPEIDNPEYSPDPSIYAYDNFGVLGLDLWQVKSGTIFDNFLITNDEAYAEEFGNETWGVTKAAEKQMKDKQDEEQRLKEEEEDKKRKEEEEAEDKEDDEDKDEDEEDEEDKEEDEEEDVPGQAKDEL

Mol* PDB structure viewerUniLectin3D

Select a PDB entry:

Structural models

AlphaFold v2: AF-P27797-F1 Download

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

AlphaFold_v2 structural model assessment through SWISS-MODEL tools.

Expression

Human Protein Atlas

GeneCards

mRNA expression in normal human tissues for CALR Gene

ProteomicsDB

References

NCBI References (10 PubMed Identifiers)

MPL overexpression induces a high level of mutant-CALR/MPL complex: a novel mechanism of ruxolitinib resistance in myeloproliferative neoplasms with CALR mutations. [34165774]

Possible Roles of Calreticulin in Uterine Decidualization and Receptivity in Rats and Humans. [34638846]

A Comparison of Two Molecular Methods for Detecting CALR Mutations in Myeloproliferative Neoplasms. [34542973]

Clinical and Laboratory Features of JAK2 V617F, CALR, and MPL Mutations in Malaysian Patients with Classical Myeloproliferative Neoplasm (MPN). [34300032]

Extracellularly Released Calreticulin Induced by Endoplasmic Reticulum Stress Impairs Syncytialization of Cytotrophoblast Model BeWo Cells. [34073978]

Human liver protein map: a reference database established by microsequencing and gel comparison. [1286669]

Microsequences of 145 proteins recorded in the two-dimensional gel protein database of normal human epidermal keratinocytes. [1286667]

The 5'-flanking region of the human calreticulin gene shares homology with the human GRP78, GRP94, and protein disulfide isomerase promoters. [1733953]

Characterization of the autoantigen calreticulin. [1919005]

In vitro interaction of a polypeptide homologous to human Ro/SS-A antigen (calreticulin) with a highly conserved amino acid sequence in the cytoplasmic domain of integrin alpha subunits. [1911778]

UniProt Main References (31 PubMed Identifiers)

Molecular cloning, expression, and chromosome 19 localization of a human Ro/SS-A autoantigen. [2332496]

Human protein factory for converting the transcriptome into an in vitro-expressed proteome,. [19054851]

The DNA sequence and biology of human chromosome 19. [15057824]

The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). [15489334]

Human placental calreticulin: purification, characterization and association with other proteins. [7841019]

Molecular characterization of human Ro/SS-A antigen. Amino terminal sequence of the protein moiety of human Ro/SS-A antigen and immunological activity of a corresponding synthetic peptide. [3260607]

Sequence similarity of calreticulin with a Ca2(+)-binding protein that co-purifies with an Ins(1,4,5)P3-sensitive Ca2+ store in HL-60 cells. [2400400]

The calcium-binding protein calreticulin is a major constituent of lytic granules in cytolytic T lymphocytes. [8418194]

A two-dimensional gel database of human colon carcinoma proteins. [9150948]

Calreticulin functions as a molecular chaperone in the biosynthesis of myeloperoxidase. [7876246]

TAP- and tapasin-dependent HLA-E surface expression correlates with the binding of an MHC class I leader peptide. [9427624]

Calreticulin associates with non-HLA-A,-B class I proteins in the human choriocarcinoma cell lines JEG-3 and BeWo. [9640257]

Calreticulin is expressed on the cell surface of activated human peripheral blood T lymphocytes in association with major histocompatibility complex class I molecules. [10358038]

HLA-F is a predominantly empty, intracellular, TAP-associated MHC class Ib protein with a restricted expression pattern. [10605026]

Calreticulin Is a receptor for nuclear export. [11149926]

Human placental calreticulin characterization of domain structure and post-translational modifications. [11322874]

Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. [19159218]

Lysine acetylation targets protein complexes and co-regulates major cellular functions. [19608861]

Structural basis of cyclophilin B binding by the calnexin/calreticulin P-domain. [20801878]

Initial characterization of the human central proteome. [21269460]

Resveratrol-induced changes of the human adipocyte secretion profile. [22905912]

Structure of the non-catalytic domain of the protein disulfide isomerase-related protein (PDIR) reveals function in protein binding. [23614004]

An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. [24275569]

N-terminome analysis of the human mitochondrial proteome. [25944712]

Landscape of the regulatory elements for lysine 2-hydroxyisobutyrylation pathway. [29192674]

Mutation in the intracellular chloride channel CLCC1 associated with autosomal recessive retinitis pigmentosa. [30157172]

Somatic mutations of calreticulin in myeloproliferative neoplasms. [24325356]

Somatic CALR mutations in myeloproliferative neoplasms with nonmutated JAK2. [24325359]

Structural framework of the GABARAP-calreticulin interface--implications for substrate binding to endoplasmic reticulum chaperones. [19154346]

X-ray structure of the human calreticulin globular domain reveals a peptide-binding area and suggests a multi-molecular mechanism. [21423620]

RNA

RNA (Transcript ID: NM_004343.4)

RNA RefSeq ID

NM_004343.4

RNA name

calreticulin

Isoforms

All isoforms of CALR containing a lectin domain

RNA Ensembl ID

ENST00000316448.10

Coding region

72..1325 (or 12938680.. 12943913)

mRNA location

12938609.. 12944489

NCBI Matured mRNA sequence 1901 NTP Download

m⁷G-5')ppp(5'-GUCCGUACUGCAGAGCCGCUGCCGGAGGGUCGUUUUAAAGGGCCCGCGCGUUGCCGCCCCCUCGGCCCGCCAUGCUGCUAUCCGUGCCGCUGCUGCUCGGCCUCCUCGGCCUGGCCGUCGCCGAGCCUGCCGUCUACUUCAAGGAGCAGUUUCUGGACGGAGACGGGUGGACUUCCCGCUGGAUCGAAUCCAAACACAAGUCAGAUUUUGGCAAAUUCGUUCUCAGUUCCGGCAAGUUCUACGGUGACGAGGAGAAAGAUAAAGGUUUGCAGACAAGCCAGGAUGCACGCUUUUAUGCUCUGUCGGCCAGUUUCGAGCCUUUCAGCAACAAAGGCCAGACGCUGGUGGUGCAGUUCACGGUGAAACAUGAGCAGAACAUCGACUGUGGGGGCGGCUAUGUGAAGCUGUUUCCUAAUAGUUUGGACCAGACAGACAUGCACGGAGACUCAGAAUACAACAUCAUGUUUGGUCCCGACAUCUGUGGCCCUGGCACCAAGAAGGUUCAUGUCAUCUUCAACUACAAGGGCAAGAACGUGCUGAUCAACAAGGACAUCCGUUGCAAGGAUGAUGAGUUUACACACCUGUACACACUGAUUGUGCGGCCAGACAACACCUAUGAGGUGAAGAUUGACAACAGCCAGGUGGAGUCCGGCUCCUUGGAAGACGAUUGGGACUUCCUGCCACCCAAGAAGAUAAAGGAUCCUGAUGCUUCAAAACCGGAAGACUGGGAUGAGCGGGCCAAGAUCGAUGAUCCCACAGACUCCAAGCCUGAGGACUGGGACAAGCCCGAGCAUAUCCCUGACCCUGAUGCUAAGAAGCCCGAGGACUGGGAUGAAGAGAUGGACGGAGAGUGGGAACCCCCAGUGAUUCAGAACCCUGAGUACAAGGGUGAGUGGAAGCCCCGGCAGAUCGACAACCCAGAUUACAAGGGCACUUGGAUCCACCCAGAAAUUGACAACCCCGAGUAUUCUCCCGAUCCCAGUAUCUAUGCCUAUGAUAACUUUGGCGUGCUGGGCCUGGACCUCUGGCAGGUCAAGUCUGGCACCAUCUUUGACAACUUCCUCAUCACCAACGAUGAGGCAUACGCUGAGGAGUUUGGCAACGAGACGUGGGGCGUAACAAAGGCAGCAGAGAAACAAAUGAAGGACAAACAGGACGAGGAGCAGAGGCUUAAGGAGGAGGAAGAAGACAAGAAACGCAAAGAGGAGGAGGAGGCAGAGGACAAGGAGGAUGAUGAGGACAAAGAUGAGGAUGAGGAGGAUGAGGAGGACAAGGAGGAAGAUGAGGAGGAAGAUGUCCCCGGCCAGGCCAAGGACGAGCUGUAGAGAGGCCUGCCUCCAGGGCUGGACUGAGGCCUGAGCGCUCCUGCCGCAGAGCUGGCCGCGCCAAAUAAUGUCUCUGUGAGACUCGAGAACUUUCAUUUUUUUCCAGGCUGGUUCGGAUUUGGGGUGGAUUUUGGUUUUGUUCCCCUCCUCCACUCUCCCCCACCCCCUCCCCGCCCUUUUUUUUUUUUUUUUUUAAACUGGUAUUUUAUCUUUGAUUCUCCUUCAGCCCUCACCCCUGGUUCUCAUCUUUCUUGAUCAACAUCUUUUCUUGCCUCUGUCCCCUUCUCUCAUCUCUUAGCUCCCCUCCAACCUGGGGGGCAGUGGUGUGGAGAAGCCACAGGCCUGAGAUUUCAUCUGCUCUCCUUCCUGGAGCCCAGAGGAGGGCAGCAGAAGGGGGUGGUGUCUCCAACCCCCCAGCACUGAGGAAGAACGGGGCUCUUCUCAUUUCACCCCUCCCUUUCUCCCCUGCCCCCAGGACUGGGCCACUUCUGGGUGGGGCAGUGGGUCCCAGAUUGGCUCACACUGAGAAUGUAAGAACUACAAACAAAAUUUCUAUUAAAUUAAAUUUUGUGUCUC-3'- Poly-A tail

Coding region

NCBI Matured mRNA sequence viewer

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More about the NCBI sViewer.

DNA

DNA (Gene ID: 811)

HGNC Gene symbol

CALR

HGNC Gene name

calreticulin

Chr. RefSeq ID

NC_000019.10

Chromosome N°

19 12938680.. 12943913

CDS location

12938680.. 12943913

DNA strand

strand +

CCDS ID

CCDS12288.1

Mouse ortholog gene

MGI:88252

Gene Variant

ClinVar, dbVar

DNA Ensembl ID

ENSG00000179218

HGNC other symbol

RO, SSA, cC1qR, CRT, FLJ26680

UCSC ID

uc002mvu.3

GeneBank

INSDC: M84739

GeneCards

1455

MIM

109091

VEGA ID

OTTHUMG00000180574

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