HumanLectome - Human Lectin Entry

Protein name

SIGLEC-3

Synonym(s)

Myeloid cell surface antigen CD33 (Sialic acid-binding Ig-like lectin 3) (Siglec-3) (gp67) (CD antigen CD33)

HGNC gene name

CD33

HGNC name

CD33 molecule

Protein_CD_name

CD33

Lectin confidence

Curated

UniLectin class

I-type lectin

Known lectin group

I-Type Lectins - CD33-related Siglecs

Lectin fold

b-sandwich / Ig-like

Glycan specificity

NeuAc / Neu5Ac(a2-6)Gal(b1-4)GlcNAc

RefSeq accession

Gene ID

UniProt accession

Ensembl protein ID

UniRef cluster ID

LectomeXplore score

0.398

Reference

10.1146/annurev-immunol-102419-035900

Other links

neXtProt, PFAM, InterPro

Protein sequence and protein families (fasta) (364 amino acids) Download

MPLLLLLPLLWAGALAMDPNFWLQVQESVTVQEGLCVLVPCTFFHPIPYYDKNSPVHGYWFREGAIISRDSPVATNKLDQEVQEETQGRFRLLGDPSRNNCSLSIVDARRRDNGSYFFRMERGSTKYSYKSPQLSVHVTDLTHRPKILIPGTLEPGHSKNLTCSVSWACEQGTPPIFSWLSAAPTSLGPRTTHSSVLIITPRPQDHGTNLTCQVKFAGAGVTTERTIQLNVTYVPQNPTTGIFPGDGSGKQETRAGVVHGAIGGAGVTALLALCLCLIFFIVKTHRRKAARTAVGRNDTHPTTGSASPKHQKKSKLHGPTETSSCSGAAPTVEMDEELHYASLNFHGMNPSKDTSTEYSEVRTQ

Mol* PDB structure viewerUniLectin3D

Select a PDB entry:

Structural models

AlphaFold v2: AF-P20138-F1 Download

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

AlphaFold_v2 structural model assessment through SWISS-MODEL tools.

Expression

Human Protein Atlas

GeneCards

mRNA expression in normal human tissues for CD33 Gene

ProteomicsDB

References

NCBI References (10 PubMed Identifiers)

Quantitative analyses of CD7, CD33, CD34, CD56, and CD123 within the FLT3-ITD/NPM1-MUT myeloblastic/monocytic bulk AML blastic populations. [34034609]

CD33M inhibits microglial phagocytosis, migration and proliferation, but the Alzheimer's disease-protective variant CD33m stimulates phagocytosis and proliferation, and inhibits adhesion. [33720433]

Reporter cell assay for human CD33 validated by specific antibodies and human iPSC-derived microglia. [34188106]

SIGLEC-3 (CD33) serves as an immune checkpoint receptor for HBV infection. [34060491]

CD33 rs3865444 as a risk factor for Parkinson's disease. [33582190]

Characterization of CD33 as a new member of the sialoadhesin family of cellular interaction molecules. [7718872]

Expression of an early myelopoietic antigen (CD33) on a subset of human umbilical cord blood-derived natural killer cells. [7505004]

Identification of potent mixed leukocyte reaction-stimulatory cells in human bone marrow. Putative differentiation stage of human blood dendritic cells. [8454872]

Expression of the myeloid differentiation antigen CD33 depends on the presence of human chromosome 19 in human-mouse hybrids. [1696442]

Isolation of a cDNA encoding CD33, a differentiation antigen of myeloid progenitor cells. [3139766]

UniProt Main References (14 PubMed Identifiers)

Genomic organization of the siglec gene locus on chromosome 19q13.4 and cloning of two new siglec pseudogenes. [11943481]

A study of CD33 (SIGLEC-3) antigen expression and function on activated human T and NK cells: two isoforms of CD33 are generated by alternative splicing. [16380601]

Complete sequencing and characterization of 21,243 full-length human cDNAs. [14702039]

The DNA sequence and biology of human chromosome 19. [15057824]

The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). [15489334]

A single N-linked glycosylation site is implicated in the regulation of ligand recognition by the I-type lectins CD22 and CD33. [8702538]

The sialoadhesin CD33 is a myeloid-specific inhibitory receptor. [10556798]

The myeloid-specific sialic acid-binding receptor, CD33, associates with the protein-tyrosine phosphatases, SHP-1 and SHP-2. [10206955]

Engagement of p75/AIRM1 or CD33 inhibits the proliferation of normal or leukemic myeloid cells. [10611343]

Myeloid specific human CD33 is an inhibitory receptor with differential ITIM function in recruiting the phosphatases SHP-1 and SHP-2. [10887109]

All isoforms of this gene containing a lectin domain

NP_001171079.1, NP_001763.3, XP_011525834.1, XP_011525833.1, XP_016882997.1, XP_016882998.1, NP_001076087.1, XP_016882999.1

RNA

RNA (Transcript ID: NM_001772.4)

RNA RefSeq ID

NM_001772.4

RNA name

CD33 molecule, transcript variant 1

Isoforms

All isoforms of CD33 containing a lectin domain

RNA Ensembl ID

ENST00000262262.5

Coding region

40..1134 (or 51225119.. 51239688)

mRNA location

51225080.. 51240016

NCBI Matured mRNA sequence 1462 NTP Download

m⁷G-5')ppp(5'-CUGCUCACACAGGAAGCCCUGGAAGCUGCUUCCUCAGACAUGCCGCUGCUGCUACUGCUGCCCCUGCUGUGGGCAGGGGCCCUGGCUAUGGAUCCAAAUUUCUGGCUGCAAGUGCAGGAGUCAGUGACGGUACAGGAGGGUUUGUGCGUCCUCGUGCCCUGCACUUUCUUCCAUCCCAUACCCUACUACGACAAGAACUCCCCAGUUCAUGGUUACUGGUUCCGGGAAGGAGCCAUUAUAUCCAGGGACUCUCCAGUGGCCACAAACAAGCUAGAUCAAGAAGUACAGGAGGAGACUCAGGGCAGAUUCCGCCUCCUUGGGGAUCCCAGUAGGAACAACUGCUCCCUGAGCAUCGUAGACGCCAGGAGGAGGGAUAAUGGUUCAUACUUCUUUCGGAUGGAGAGAGGAAGUACCAAAUACAGUUACAAAUCUCCCCAGCUCUCUGUGCAUGUGACAGACUUGACCCACAGGCCCAAAAUCCUCAUCCCUGGCACUCUAGAACCCGGCCACUCCAAAAACCUGACCUGCUCUGUGUCCUGGGCCUGUGAGCAGGGAACACCCCCGAUCUUCUCCUGGUUGUCAGCUGCCCCCACCUCCCUGGGCCCCAGGACUACUCACUCCUCGGUGCUCAUAAUCACCCCACGGCCCCAGGACCACGGCACCAACCUGACCUGUCAGGUGAAGUUCGCUGGAGCUGGUGUGACUACGGAGAGAACCAUCCAGCUCAACGUCACCUAUGUUCCACAGAACCCAACAACUGGUAUCUUUCCAGGAGAUGGCUCAGGGAAACAAGAGACCAGAGCAGGAGUGGUUCAUGGGGCCAUUGGAGGAGCUGGUGUUACAGCCCUGCUCGCUCUUUGUCUCUGCCUCAUCUUCUUCAUAGUGAAGACCCACAGGAGGAAAGCAGCCAGGACAGCAGUGGGCAGGAAUGACACCCACCCUACCACAGGGUCAGCCUCCCCGAAACACCAGAAGAAGUCCAAGUUACAUGGCCCCACUGAAACCUCAAGCUGUUCAGGUGCCGCCCCUACUGUGGAGAUGGAUGAGGAGCUGCAUUAUGCUUCCCUCAACUUUCAUGGGAUGAAUCCUUCCAAGGACACCUCCACCGAAUACUCAGAGGUCAGGACCCAGUGAGGAACCCACAAGAGCAUCAGGCUCAGCUAGAAGAUCCACAUCCUCUACAGGUCGGGGACCAAAGGCUGAUUCUUGGAGAUUUAACACCCCACAGGCAAUGGGUUUAUAGACAUUAUGUGAGUUUCCUGCUAUAUUAACAUCAUCUUAGACUUUGCAAGCAGAGAGUCGUGGAAUCAAAUCUGUGCUCUUUCAUUUGCUAAGUGUAUGAUGUCACACAAGCUCCUUAACCUUCCAUGUCUCCAUUUUCUUCUCUGUGAAGUAGGUAUAAGAAGUCCUAUCUCAUAGGGAUGCUGUGAGCAUUAAAUAAAGGUACACAUGGAAAACACCA-3'- Poly-A tail

Coding region

NCBI Matured mRNA sequence viewer

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More about the NCBI sViewer.

DNA

DNA (Gene ID: 945)

HGNC Gene symbol

CD33

HGNC Gene name

CD33 molecule

Chr. RefSeq ID

NC_000019.10

Chromosome N°

19 51225119.. 51239688

CDS location

51225119.. 51239688

DNA strand

strand +

CCDS ID

CCDS33084.1

Mouse ortholog gene

MGI:99440

Gene Variant

ClinVar, dbVar

DNA Ensembl ID

ENSG00000105383

HGNC other symbol

SIGLEC3, SIGLEC-3, p67, FLJ00391

UCSC ID

uc002pwa.3

GeneBank

INSDC: M23197

GeneCards

1659

MIM

159590

VEGA ID

OTTHUMG00000182891

Other links

ENA, DDBJ, Monarch

CD gene name

CD33

NCBI CDS Gene viewer

More about the NCBI sViewer.

NCBI CDS gene sequence (1095 bp)

5'-ATGCCGCTGCTGCTACTGCTGCCCCTGCTGTGGGCAGGGGCCCTGGCTATGGATCCAAATTTCTGGCTGCAAGTGCAGGAGTCAGTGACGGTACAGGAGGGTTTGTGCGTCCTCGTGCCCTGCACTTTCTTCCATCCCATACCCTACTACGACAAGAACTCCCCAGTTCATGGTTACTGGTTCCGGGAAGGAGCCATTATATCCAGGGACTCTCCAGTGGCCACAAACAAGCTAGATCAAGAAGTACAGGAGGAGACTCAGGGCAGATTCCGCCTCCTTGGGGATCCCAGTAGGAACAACTGCTCCCTGAGCATCGTAGACGCCAGGAGGAGGGATAATGGTTCATACTTCTTTCGGATGGAGAGAGGAAGTACCAAATACAGTTACAAATCTCCCCAGCTCTCTGTGCATGTGACAGACTTGACCCACAGGCCCAAAATCCTCATCCCTGGCACTCTAGAACCCGGCCACTCCAAAAACCTGACCTGCTCTGTGTCCTGGGCCTGTGAGCAGGGAACACCCCCGATCTTCTCCTGGTTGTCAGCTGCCCCCACCTCCCTGGGCCCCAGGACTACTCACTCCTCGGTGCTCATAATCACCCCACGGCCCCAGGACCACGGCACCAACCTGACCTGTCAGGTGAAGTTCGCTGGAGCTGGTGTGACTACGGAGAGAACCATCCAGCTCAACGTCACCTATGTTCCACAGAACCCAACAACTGGTATCTTTCCAGGAGATGGCTCAGGGAAACAAGAGACCAGAGCAGGAGTGGTTCATGGGGCCATTGGAGGAGCTGGTGTTACAGCCCTGCTCGCTCTTTGTCTCTGCCTCATCTTCTTCATAGTGAAGACCCACAGGAGGAAAGCAGCCAGGACAGCAGTGGGCAGGAATGACACCCACCCTACCACAGGGTCAGCCTCCCCGAAACACCAGAAGAAGTCCAAGTTACATGGCCCCACTGAAACCTCAAGCTGTTCAGGTGCCGCCCCTACTGTGGAGATGGATGAGGAGCTGCATTATGCTTCCCTCAACTTTCATGGGATGAATCCTTCCAAGGACACCTCCACCGAATACTCAGAGGTCAGGACCCAGTGA-3'

NCBI CDS gene sequence with introns (location: 51225119.. 51239688) (14570 bp)Download

NCBI Gene Viewer (CDS + introns)

NCBI CDS gene sequence with introns, 5'UTR and 3'UTR (location: 51225080.. 51240016) (14937 bp)Download

NCBI Gene Viewer (CDS + introns + Untranslated regions)

NCBI gene sequence (location: [51225080 - 1000].. 51240016) (15937 bp)Download

NCBI Gene Viewer (CDS + introns + Untranslated regions + 1000 dNTP to potentially reach the promotor)

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