NCBI Summary
Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer, and affect healing of the epithelium. This gene, which is expressed in the gastric mucosa, has also been studied because of its expression in human tumors. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq, Jul 2008].
Protein
Protein (NP_003216)
Trefoil factor 1
Trefoil factor 1 (Breast cancer estrogen-inducible protein) (PNR-2) (Polypeptide P1.A) (hP1.A) (Protein pS2)
TFF1
trefoil factor 1
Undefined
Curated
Trefoil Factor
Undefined
peptide
GlcNAc(a1-4)Gal
NP_003216
7031
P04155
ENSP00000291527.2
P04155
0.79
10.1038/s41467-020-16223-7
neXtProt, PFAM, InterPro
Protein sequence and protein families (fasta) (84 amino acids) Download
MATMENKVICALVLVSMLALGTLAEAQTETCTVAPRERQNCGFPGVTPSQCANKGCCFDDTVRGVPWCFYPNTIDVPPEEECEF
Mol* PDB structure viewerUniLectin3D
Structural models
AlphaFold v2: AF-P04155-F1Download
  Model Confidence:
  •    Very high (pLDDT > 90)
  •    Confident (90 > pLDDT > 70)
  •    Low (70 > pLDDT > 50)
  •    Very low (pLDDT < 50)

  AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Ligand
Glycan ligands from structural data
No crystal structures of complexes with glycan ligand.
Expression
Human Protein Atlas
GeneCards
mRNA expression in normal human tissues for TFF1 Gene
ProteomicsDB
References
NCBI References (10 PubMed Identifiers)
  • Estrogen-related receptor-gamma influences Helicobacter pylori infection by regulating TFF1 in gastric cancer. [34058470]
  • Trefoil factor-1 upregulation in estrogen-receptor positive breast cancer correlates with an increased risk of bone metastasis. [33249323]
  • TFF1 Induces Aggregation and Reduces Motility of Helicobacter pylori. [33673347]
  • Chemical synthesis of human trefoil factor 1 (TFF1) and its homodimer provides novel insights into their mechanisms of action. [32391824]
  • Trefoil factors share a lectin activity that defines their role in mucus. [32404934]
  • Antipeptide antibodies against the pNR-2 oestrogen-regulated protein of human breast cancer cells and detection of pNR-2 expression in normal tissues by immunohistochemistry. [1707960]
  • Expression of the pS2 gene in human gastric cancer cells derived from poorly differentiated adenocarcinoma. [2311759]
  • hSP, the domain-duplicated homolog of pS2 protein, is co-expressed with pS2 in stomach but not in breast carcinoma. [2303034]
  • Complete primary structure of the human estrogen-responsive gene (pS2) product. [2185238]
  • Breast cancer-associated pS2 protein: synthesis and secretion by normal stomach mucosa. [3041593]
UniProt Main References (18 PubMed Identifiers)
  • Sequence of the pS2 mRNA induced by estrogen in the human breast cancer cell line MCF-7. [6324130]
  • Cloning of a gene expressed in human breast cancer and regulated by estrogen in MCF-7 cells. [3838275]
  • Structure of the human oestrogen-responsive gene pS2. [3822834]
  • Refined localization of autosomal recessive nonsyndromic deafness DFNB10 locus using 34 novel microsatellite markers, genomic structure, and exclusion of six known genes in the region. [10950923]
  • The DNA sequence of human chromosome 21. [10830953]
  • The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). [15489334]
  • [Primary structure of human protein pS2]. [3146413]
  • Interaction between TFF1, a gastric tumor suppressor trefoil protein, and TFIZ1, a brichos domain-containing protein with homology to SP-C. [15924415]
  • Identification of human urinary trefoil factor 1 as a novel calcium oxalate crystal growth inhibitor. [16308573]
  • Identification of a polypeptide secreted by human breast cancer cells (MCF-7) as the human estrogen-responsive gene (pS2) product. [3261981]
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RNA
RNA (Transcript ID: NM_003225.3)
NM_003225.3
trefoil factor 1
All isoforms of TFF1 containing a lectin domain
ENST00000291527.3
41..295 (or 42362479.. 42366495)
42362282.. 42366535
NCBI Matured mRNA sequence 492 NTPDownload 
m7G-5')ppp(5'-AUCCCUGACUCGGGGUCGCCUUUGGAGCAGAGAGGAGGCAAUGGCCACCAUGGAGAACAAGGUGAUCUGCGCCCUGGUCCUGGUGUCCAUGCUGGCCCUCGGCACCCUGGCCGAGGCCCAGACAGAGACGUGUACAGUGGCCCCCCGUGAAAGACAGAAUUGUGGUUUUCCUGGUGUCACGCCCUCCCAGUGUGCAAAUAAGGGCUGCUGUUUCGACGACACCGUUCGUGGGGUCCCCUGGUGCUUCUAUCCUAAUACCAUCGACGUCCCUCCAGAAGAGGAGUGUGAAUUUUAGACACUUCUGCAGGGAUCUGCCUGCAUCCUGACGCGGUGCCGUCCCCAGCACGGUGAUUAGUCCCAGAGCUCGGCUGCCACCUCCACCGGACACCUCAGACACGCUUCUGCAGCUGUGCCUCGGCUCACAACACAGAUUGACUGCUCUGACUUUGACUACUCAAAAUUGGCCUAAAAAUUAAAAGAGAUCGAUAUUAA-3'- Poly-A tail
  • Coding region
NCBI Matured mRNA sequence viewer
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More about the NCBI sViewer.
DNA
DNA (Gene ID: 7031)
TFF1
trefoil factor 1
NC_000021.9
21  42362479.. 42366495
42362479.. 42366495
strand -
CCDS13685.1
MGI:88135
ClinVar, dbVar
ENSG00000160182
D21S21, HPS2, pS2, pNR-2, HP1.A
uc002zax.2
INSDC: BC032811
11755
113710
OTTHUMG00000086799
ENA, DDBJ, Monarch
NCBI CDS Gene viewer
More about the NCBI sViewer.
NCBI CDS gene sequence (255 bp)
5'-ATGGCCACCATGGAGAACAAGGTGATCTGCGCCCTGGTCCTGGTGTCCATGCTGGCCCTCGGCACCCTGGCCGAGGCCCAGACAGAGACGTGTACAGTGGCCCCCCGTGAAAGACAGAATTGTGGTTTTCCTGGTGTCACGCCCTCCCAGTGTGCAAATAAGGGCTGCTGTTTCGACGACACCGTTCGTGGGGTCCCCTGGTGCTTCTATCCTAATACCATCGACGTCCCTCCAGAAGAGGAGTGTGAATTTTAG-3'
NCBI CDS gene sequence with introns (location: 42362479.. 42366495) (4017 bp)Download
NCBI Gene Viewer (CDS + introns)
NCBI CDS gene sequence with introns, 5'UTR and 3'UTR (location: 42362282.. 42366535) (4254 bp)Download
NCBI Gene Viewer (CDS + introns + Untranslated regions)
NCBI gene sequence (location: [42362282.. 42366535 + 1000]) (5254 bp)Download
NCBI Gene Viewer (CDS + introns + Untranslated regions + 1000 dNTP to potentially reach the promotor)
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