NCBI Summary
This gene encodes a protein with similarity to a bovine microfibril-associated protein. The protein has binding specificities for both collagen and carbohydrate. It is thought to be an extracellular matrix protein which is involved in cell adhesion or intercellular interactions. The gene is located within the Smith-Magenis syndrome region. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010].
Protein
Protein (NP_002395)
Microfibril-associated glycoprotein 4
Microfibril-associated glycoprotein 4
MFAP4
microfibril associated protein 4
Undefined
Very low evidence
Ficolin-like
Undefined
a/b mixed with b-sheet / Fibrinogen C-ter like
Uncharacterised
NP_002395
4239
P55083
ENSP00000299610.5
P55083
0.623
Undefined
neXtProt, PFAM, InterPro
Protein sequence and protein families (fasta) (255 amino acids) Download
MKALLALPLLLLLSTPPCAPQVSGIRGDALERFCLQQPLDCDDIYAQGYQSDGVYLIYPSGPSVPVPVFCDMTTEGGKWTVFQKRFNGSVSFFRGWNDYKLGFGRADGEYWLGLQNMHLLTLKQKYELRVDLEDFENNTAYAKYADFSISPNAVSAEEDGYTLFVAGFEDGGAGDSLSYHSGQKFSTFDRDQDLFVQNCAALSSGAFWFRSCHFANLNGFYLGGSHLSYANGINWAQWKGFYYSLKRTEMKIRRA
No structure currently available in the PDB RCSB Databank.
Structural models
AlphaFold v2: AF-P55083-F1Download
  Model Confidence:
  •    Very high (pLDDT > 90)
  •    Confident (90 > pLDDT > 70)
  •    Low (70 > pLDDT > 50)
  •    Very low (pLDDT < 50)

  AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

SWISS-MODEL structural models
SWISS-MODEL structural model
Modeller structural model (Homology modelling pipeline), Error: [1.17, 1.42] ÅDownload
The location of the lectin domain structural model is: 32-255
We infer [1.17, 1.42] Å as the interval of error of this structural model.
Template 1: 6ZQX chain: A, Q8N539, NP_116232.3, sequence identity: 54.0%, coverage: 97.8%, location in sequence: 239-457, (239-457 in PDB).
Template 2: 2J3U chain: B, Q15485, NP_004099.2, sequence identity: 48.7%, coverage: 97.3%, location in sequence: 96-313, (71-288 in PDB).
Template 3: 2JHK chain: F, O00602, NP_001994.2, sequence identity: 47.8%, coverage: 96.9%, location in sequence: 110-326, (81-297 in PDB).
Show the alignment used for the construction of the structural model, Download.
Show the plot of DOPE energy score, Download.
Ligand
Glycan ligands from structural data
No crystal structures of complexes with glycan ligand.
Expression
Human Protein Atlas
GeneCards
mRNA expression in normal human tissues for MFAP4 Gene
ProteomicsDB
References
NCBI References (10 PubMed Identifiers)
  • Microfibril associated protein 4 (MFAP4) is a carrier of the tumor associated carbohydrate sialyl-Lewis x (sLex) in pancreatic adenocarcinoma. [33038510]
  • Human Microfibrillar-Associated Protein 4 (MFAP4) Gene Promoter: A TATA-Less Promoter That Is Regulated by Retinol and Coenzyme Q10 in Human Fibroblast Cells. [33182307]
  • The fibrinogen C-terminal domain is seldom C-mannosylated but its C-mannosylation is important for the secretion of microfibril-associated glycoprotein 4. [32442478]
  • High plasma microfibrillar-associated protein 4 is associated with reduced surgical repair in abdominal aortic aneurysms. [31784280]
  • Relationship between Microfibrillar-Associated Protein 4 Levels and Subclinical Myocardial Damage in Chronic Kidney Disease. [32268335]
  • Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry. [16335952]
  • Signal peptide prediction based on analysis of experimentally verified cleavage sites. [15340161]
  • Microfibril-associated protein 4 is present in lung washings and binds to the collagen region of lung surfactant protein D. [10542261]
  • Ultrastructural distribution of 36-kD microfibril-associated glycoprotein (MAGP-36) in human and bovine tissues. [10424889]
  • The gene for a human microfibril-associated glycoprotein is commonly deleted in Smith-Magenis syndrome patients. [7633408]
UniProt Main References (5 PubMed Identifiers)
  • Complete sequencing and characterization of 21,243 full-length human cDNAs. [14702039]
  • DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage. [16625196]
  • The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). [15489334]
  • Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. [19159218]
  • Characterization of Microfibrillar-associated Protein 4 (MFAP4) as a Tropoelastin- and Fibrillin-binding Protein Involved in Elastic Fiber Formation. [26601954]
All isoforms of this gene containing a lectin domain
NP_002395.1, NP_001185624.1
RNA
RNA (Transcript ID: NM_002404.3)
NM_002404.3
microfibril associated protein 4, transcript variant 2
All isoforms of MFAP4 containing a lectin domain
ENST00000299610.5
36..803 (or 19384462.. 19387155)
19383445.. 19387190
NCBI Matured mRNA sequence 1820 NTPDownload 
m7G-5')ppp(5'-GCAGACACCCAGCCACUCUGAGCAGAACUGACAGCAUGAAGGCACUCCUGGCCCUGCCGCUGCUGCUGCUUCUCUCCACGCCCCCGUGUGCCCCCCAGGUCUCCGGGAUCCGAGGAGAUGCUCUGGAGAGGUUUUGCCUUCAGCAACCCCUGGACUGUGACGACAUCUAUGCCCAGGGCUACCAGUCAGACGGCGUGUACCUCAUCUACCCCUCGGGCCCCAGUGUGCCUGUGCCCGUCUUCUGUGACAUGACCACCGAGGGCGGGAAGUGGACGGUUUUCCAGAAGAGAUUCAAUGGCUCAGUAAGUUUCUUCCGCGGCUGGAAUGACUACAAGCUGGGCUUCGGCCGUGCUGAUGGAGAGUACUGGCUGGGGCUGCAGAACAUGCACCUCCUGACACUGAAGCAGAAGUAUGAGCUGCGAGUGGACUUGGAGGACUUUGAGAACAACACGGCCUAUGCCAAGUACGCUGACUUCUCCAUCUCCCCGAACGCGGUCAGCGCAGAGGAGGAUGGCUACACCCUCUUUGUGGCAGGCUUUGAGGAUGGCGGGGCAGGUGACUCCCUGUCCUACCACAGUGGCCAGAAGUUCUCUACCUUCGACCGGGACCAGGACCUCUUUGUGCAGAACUGCGCAGCUCUCUCCUCAGGAGCCUUCUGGUUCCGCAGCUGCCACUUUGCCAACCUCAAUGGCUUCUACCUAGGUGGCUCCCACCUCUCUUAUGCCAAUGGCAUCAACUGGGCCCAGUGGAAGGGCUUCUACUACUCCCUCAAACGCACUGAGAUGAAAAUCCGCCGGGCCUGAAGGGCUGGCCCCCUCAGGCACCUUUCCUCCCCUGGACACCCAUGGUCUCCAUGAGUGCUCCCUCUGCUGCCCCUGAUGCAUGCUUCUGCUGAUUCCCGAGCACCAACUCCUUACAAGGGGGCCUUGUGGCUCUCAGCCAUGCCACAUCCCUGUCACACACCCAGGGCAUCCAUUCCUAAGCCAGACCCGGCUCCCCUACACCUGAAGUUACACUGCCAGCAGUUCCCCAGGCCUCUUCCGAGAGGCACAUGGUUCUAGCCUGGACCUGGCUGGGCUCCAUGAGAAUGAGUUGCCUCCAACCUGUCCCAACAGCUGACAGCCAGGAGCCACUCUCCCAGCUGCAGGCCUUUGUGGUCCAUCUUGUCCUGCUUCCUCACUGUGGACCCCUGUCUGGGCCACCCUAGUGUGCUAAGCUGAGCAGUGCAGUGUGAACAGGGCCCAUGGUGUAUUCUAGGCCACAGCCCAGCACUCCUCUGGGCUGCUCUCAAACCAUGUCCCAUCUUCAGCAUCCCUCCCACCAACUUACUCCCCUGUGGUGAGUACCGUGGAACCCCAGCCCACCUCACUAUCAUACUCAGCUUCCCCUGAUGGCCCAUCCCAGCCCCUGAAGCUCUAUGCCAAGAACACAGCUACCGCACACCACCCUGAAACAGCCACAGCCAAGGUAGGCAUGCAUAUGAGGUCUUCCCCAUACCCUCUGGGUGUUGAGAGGUUUAGCCACAUGAGGGAGCAGAGGACAAUCUCUGCAGGGCUGGGAGUGGGUAGGGACUGAAGGUCUCAAUAAACCUUCAGAACCUGAAUGAACUGGCUUCAUACACACAAACAUAUUUGUUUAUCCCCCAAAUGUAGGCACCUGGCUCCUCCUUGCUCCCCUGCUGAUGGUGUCCUACCCCGAACUCCAAAAAUUACACCUGGAGUCAGGUGCAGAAGGGAACCUUGUAUUUCACAGGCCUCAUUUUGAUGGCAAAAAGACAGUGUAAUAAUAACAUAAUAAUAAUAAAAAUAUAAUACUGAAAA-3'- Poly-A tail
  • Coding region
NCBI Matured mRNA sequence viewer
;
More about the NCBI sViewer.
DNA
DNA (Gene ID: 4239)
MFAP4
microfibril associated protein 4
NC_000017.11
17  19384462.. 19387155
19384462.. 19387155
strand -
CCDS11208.1
MGI:1342276
ClinVar, dbVar
ENSG00000166482
uc002gvt.4
INSDC: L38486
7035
600596
OTTHUMG00000059585
ENA, DDBJ, Monarch
NCBI CDS Gene viewer
More about the NCBI sViewer.
NCBI CDS gene sequence (768 bp)
5'-ATGAAGGCACTCCTGGCCCTGCCGCTGCTGCTGCTTCTCTCCACGCCCCCGTGTGCCCCCCAGGTCTCCGGGATCCGAGGAGATGCTCTGGAGAGGTTTTGCCTTCAGCAACCCCTGGACTGTGACGACATCTATGCCCAGGGCTACCAGTCAGACGGCGTGTACCTCATCTACCCCTCGGGCCCCAGTGTGCCTGTGCCCGTCTTCTGTGACATGACCACCGAGGGCGGGAAGTGGACGGTTTTCCAGAAGAGATTCAATGGCTCAGTAAGTTTCTTCCGCGGCTGGAATGACTACAAGCTGGGCTTCGGCCGTGCTGATGGAGAGTACTGGCTGGGGCTGCAGAACATGCACCTCCTGACACTGAAGCAGAAGTATGAGCTGCGAGTGGACTTGGAGGACTTTGAGAACAACACGGCCTATGCCAAGTACGCTGACTTCTCCATCTCCCCGAACGCGGTCAGCGCAGAGGAGGATGGCTACACCCTCTTTGTGGCAGGCTTTGAGGATGGCGGGGCAGGTGACTCCCTGTCCTACCACAGTGGCCAGAAGTTCTCTACCTTCGACCGGGACCAGGACCTCTTTGTGCAGAACTGCGCAGCTCTCTCCTCAGGAGCCTTCTGGTTCCGCAGCTGCCACTTTGCCAACCTCAATGGCTTCTACCTAGGTGGCTCCCACCTCTCTTATGCCAATGGCATCAACTGGGCCCAGTGGAAGGGCTTCTACTACTCCCTCAAACGCACTGAGATGAAAATCCGCCGGGCCTGA-3'
NCBI CDS gene sequence with introns (location: 19384462.. 19387155) (2694 bp)Download
NCBI Gene Viewer (CDS + introns)
NCBI CDS gene sequence with introns, 5'UTR and 3'UTR (location: 19383445.. 19387190) (3746 bp)Download
NCBI Gene Viewer (CDS + introns + Untranslated regions)
NCBI gene sequence (location: [19383445.. 19387190 + 1000]) (4746 bp)Download
NCBI Gene Viewer (CDS + introns + Untranslated regions + 1000 dNTP to potentially reach the promotor)
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