HumanLectome - Human Lectin Entry

NCBI Summary

This gene encodes a protein that is highly expressed in osteosarcomas. This protein binds to the hypoxia-inducible factor 1 (HIF-1), a key regulator of the hypoxic response and angiogenesis, and promotes the degradation of one of its subunits. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008].

Protein name

MRH - mannose-6-phosphate receptor homology domain

Synonym(s)

Protein OS-9 (Amplified in osteosarcoma 9)

HGNC gene name

OS9

HGNC name

OS9 endoplasmic reticulum lectin

Protein_CD_name

Undefined

Lectin confidence

Curated

UniLectin class

P-type lectin-like

Known lectin group

P-typeLectins

Lectin fold

b-barrel

Glycan specificity

Man(a1-3)[Man(a1-6)][Man(a1-6)][Man(a1-3)]Man

RefSeq accession

Gene ID

UniProt accession

Ensembl protein ID

UniRef cluster ID

LectomeXplore score

1.0

Reference

10.1016/j.molcel.2010.11.017

Other links

neXtProt, PFAM, InterPro

Protein sequence and protein families (fasta) (667 amino acids) Download

MAAETLLSSLLGLLLLGLLLPASLTGGVGSLNLEELSEMRYGIEILPLPVMGGQSQSSDVVIVSSKYKQRYECRLPAGAIHFQREREEETPAYQGPGIPELLSPMRDAPCLLKTKDWWTYEFCYGRHIQQYHMEDSEIKGEVLYLGYYQSAFDWDDETAKASKQHRLKRYHSQTYGNGSKCDLNGRPREAEVRFLCDEGAGISGDYIDRVDEPLSCSYVLTIRTPRLCPHPLLRPPPSAAPQAILCHPSLQPEEYMAYVQRQADSKQYGDKIIEELQDLGPQVWSETKSGVAPQKMAGASPTKDDSKDSDFWKMLNEPEDQAPGGEEVPAEEQDPSPEAADSASGAPNDFQNNVQVKVIRSPADLIRFIEELKGGTKKGKPNIGQEQPVDDAAEVPQREPEKERGDPERQREMEEEEDEDEDEDEDEDERQLLGEFEKELEGILLPSDRDRLRSEVKAGMERELENIIQETEKELDPDGLKKESERDRAMLALTSTLNKLIKRLEEKQSPELVKKHKKKRVVPKKPPPSPQPTEEDPEHRVRVRVTKLRLGGPNQDLTVLEMKRENPQLKQIEGLVKELLEREGLTAAGKIEIKIVRPWAEGTEEGARWLTDEDTRNLKEIFFNILVPGAEEAQKERQRQKELESNYRRVWGSPGGEGTGDLDEFDF

Mol* PDB structure viewerUniLectin3D

Select a PDB entry:

Structural models

AlphaFold v2: AF-Q13438-F1 Download

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

AlphaFold_v2 structural model assessment through SWISS-MODEL tools.

Expression

Human Protein Atlas

GeneCards

mRNA expression in normal human tissues for OS9 Gene

ProteomicsDB

References

NCBI References (10 PubMed Identifiers)

Monochorionic twins with selective fetal growth restriction: insight from placental whole-transcriptome analysis. [32437666]

Redundant and Antagonistic Roles of XTP3B and OS9 in Decoding Glycan and Non-glycan Degrons in ER-Associated Degradation. [29706535]

The endoplasmic reticulum-associated protein, OS-9, behaves as a lectin in targeting the immature calcium-sensing receptor. [28419469]

Conserved cytoplasmic domains promote Hrd1 ubiquitin ligase complex formation for ER-associated degradation (ERAD). [28827405]

Substrate-Trapped Interactors of PHD3 and FIH Cluster in Distinct Signaling Pathways. [26972000]

Cloning and characterization of three isoforms of OS-9 cDNA and expression of the OS-9 gene in various human tumor cell lines. [9562620]

Genomic organization of the OS-9 gene amplified in human sarcomas. [9498564]

Transcript mapping in a 46-kb sequenced region at the core of 12q13.3 amplification in human cancers. [9192850]

Complete sequence analysis of a gene (OS-9) ubiquitously expressed in human tissues and amplified in sarcomas. [8634085]

Direct isolation of genes encoded within a homogeneously staining region by chromosome microdissection. [8090779]

UniProt Main References (14 PubMed Identifiers)

Complete sequencing and characterization of 21,243 full-length human cDNAs. [14702039]

The finished DNA sequence of human chromosome 12. [16541075]

The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). [15489334]

Ca2(+)-dependent interaction of N-copine, a member of the two C2 domain protein family, with OS-9, the product of a gene frequently amplified in osteosarcoma. [10403379]

OS-9 regulates the transit and polyubiquitination of TRPV4 in the endoplasmic reticulum. [17932042]

A dual task for the Xbp1-responsive OS-9 variants in the mammalian endoplasmic reticulum: inhibiting secretion of misfolded protein conformers and enhancing their disposal. [18417469]

Human XTP3-B forms an endoplasmic reticulum quality control scaffold with the HRD1-SEL1L ubiquitin ligase complex and BiP. [18502753]

OS-9 and GRP94 deliver mutant alpha1-antitrypsin to the Hrd1-SEL1L ubiquitin ligase complex for ERAD. [18264092]

Human OS-9, a lectin required for glycoprotein endoplasmic reticulum-associated degradation, recognizes mannose-trimmed N-glycans. [19346256]

Mammalian OS-9 is upregulated in response to endoplasmic reticulum stress and facilitates ubiquitination of misfolded glycoproteins. [19084021]

All isoforms of this gene containing a lectin domain

NP_001017957.1, XP_006719264.1, NP_001017956.1, NP_001248349.1, NP_001017958.1, XP_006719263.1, NP_006803.1, XP_005268638.1, NP_001248352.1, NP_001248350.1, NP_001248351.1

RNA

RNA (Transcript ID: NM_006812.4)

RNA RefSeq ID

NM_006812.4

RNA name

OS9 endoplasmic reticulum lectin, transcript variant 1

Isoforms

All isoforms of OS9 containing a lectin domain

RNA Ensembl ID

ENST00000315970.12

Coding region

31..2034 (or 57694162.. 57720909)

mRNA location

57694132.. 57721557

NCBI Matured mRNA sequence 2682 NTP Download

m⁷G-5')ppp(5'-AGAUUCUCUGCAUAAGAAGGGGAACGAAAGAUGGCGGCGGAAACGCUGCUGUCCAGUUUGUUAGGACUGCUGCUUCUGGGACUCCUGUUACCCGCAAGUCUGACCGGCGGUGUCGGGAGCCUGAACCUGGAGGAGCUGAGUGAGAUGCGUUAUGGGAUCGAGAUCCUGCCGUUGCCUGUCAUGGGAGGGCAGAGCCAAUCUUCGGACGUGGUGAUUGUCUCCUCUAAGUACAAACAGCGCUAUGAGUGUCGCCUGCCAGCUGGAGCUAUUCACUUCCAGCGUGAAAGGGAGGAGGAAACACCUGCUUACCAAGGGCCUGGGAUCCCUGAGUUGUUGAGCCCAAUGAGAGAUGCUCCCUGCUUGCUGAAGACAAAGGACUGGUGGACAUAUGAAUUCUGUUAUGGACGCCACAUCCAGCAAUACCACAUGGAAGAUUCAGAGAUCAAAGGUGAAGUCCUCUAUCUCGGCUACUACCAAUCAGCCUUCGACUGGGAUGAUGAAACAGCCAAGGCCUCCAAGCAGCAUCGUCUUAAACGCUACCACAGCCAGACCUAUGGCAAUGGGUCCAAGUGCGACCUUAAUGGGAGGCCCCGGGAGGCCGAGGUUCGGUUCCUCUGUGACGAGGGUGCAGGUAUCUCUGGGGACUACAUCGAUCGCGUGGACGAGCCCUUGUCCUGCUCUUAUGUGCUGACCAUUCGCACUCCUCGGCUCUGCCCCCACCCUCUCCUCCGGCCCCCACCCAGUGCUGCACCGCAGGCCAUCCUCUGUCACCCUUCCCUACAGCCUGAGGAGUACAUGGCCUACGUUCAGAGGCAAGCCGACUCAAAGCAGUAUGGAGAUAAAAUCAUAGAGGAGCUGCAAGAUCUAGGCCCCCAAGUGUGGAGUGAGACCAAGUCUGGGGUGGCACCCCAAAAGAUGGCAGGUGCGAGCCCGACCAAGGAUGACAGUAAGGACUCAGAUUUCUGGAAGAUGCUUAAUGAGCCAGAGGACCAGGCCCCAGGAGGGGAGGAGGUGCCGGCUGAGGAGCAGGACCCAAGCCCUGAGGCAGCAGAUUCAGCUUCUGGUGCUCCCAAUGAUUUUCAGAACAACGUGCAGGUCAAAGUCAUUCGAAGCCCUGCGGAUUUGAUUCGAUUCAUAGAGGAGCUGAAAGGUGGAACAAAAAAGGGGAAGCCAAAUAUAGGCCAAGAGCAGCCUGUGGAUGAUGCUGCAGAAGUCCCUCAGAGGGAACCAGAGAAGGAAAGGGGUGAUCCAGAACGGCAGAGAGAGAUGGAAGAAGAGGAGGAUGAGGAUGAGGAUGAGGAUGAAGAUGAGGAUGAACGGCAGUUACUGGGAGAAUUUGAGAAGGAACUGGAAGGGAUCCUGCUUCCGUCAGACCGAGACCGGCUCCGUUCGGAGGUGAAGGCUGGCAUGGAGCGGGAACUGGAAAACAUCAUCCAGGAGACAGAGAAAGAGCUGGACCCAGAUGGGCUGAAGAAGGAGUCAGAGCGGGAUCGGGCAAUGCUGGCUCUCACAUCCACUCUCAACAAACUCAUCAAAAGACUGGAGGAAAAACAGAGUCCAGAGCUGGUGAAGAAGCACAAGAAAAAGAGGGUUGUCCCCAAAAAGCCUCCCCCAUCACCCCAACCUACAGAGGAGGAUCCUGAGCACAGAGUCCGGGUCCGGGUCACCAAGCUCCGUCUCGGAGGCCCUAAUCAGGAUCUGACUGUCCUCGAGAUGAAACGGGAAAACCCACAGCUGAAACAAAUCGAGGGGCUGGUGAAGGAGCUGCUGGAGAGGGAGGGACUCACAGCUGCAGGGAAAAUUGAGAUCAAAAUUGUCCGCCCAUGGGCUGAAGGGACUGAAGAGGGUGCACGUUGGCUGACUGAUGAGGACACGAGAAACCUCAAGGAGAUCUUCUUCAAUAUCUUGGUGCCGGGAGCUGAAGAGGCCCAGAAGGAACGCCAGCGGCAGAAAGAGCUGGAGAGCAAUUACCGCCGGGUGUGGGGCUCUCCAGGUGGGGAGGGCACAGGGGACCUGGACGAAUUUGACUUCUGAGACCAACACUACACUUGACCCUUCACGGAAUCCAGACUCUUCCUGGACUGGCUUGCCUCCUCCCCACCUCCCCACCCUGGAACCCCUGAGGGCCAAACAGCAGAGUGGAGCUGAGCUGUGGACCUCUCGGGCAACUCUGUGGGUGUGGGGGCCCUGGGUGAAUGCUGCUGCCCCUGCUGGCAGCCACCUUGAGACCUCACCGGGCCUGUGAUAUUUGCUCUCCUGAACUCUCACUCAAUCCUCUUCCUCUCCUCUGUGGCUUUUCCUGUUAUUGUCCCCUAAUGAUAGGAUAUUCCCUGCUGCCUACCUGGAGAUUCAGUAGGAUCUUUUGAGUGGAGGUGGGUAGAGAGAGCAAGGAGGGCAGGACACUUAGCAGGCACUGAGCAAGCAGGCCCCCACCUGCCCUUAGUGAUGUUUGGAGUCGUUUUACCCUCUUCUAUUGAAUUGCCUUGGGAUUUCCUUCUCCCUUUCCCUGCCCACCCUGUCCCCUACAAUUUGUGCUUCUGAGUUGAGGAGCCUUCACCUCUGUUGCUGAGGAAAUGGUAGAAUGCUGCCUAUCACCUCCAGCACAAUCCCAGUGAAAAAGGUGUGAAGCACCCACCAUGUUCUUGAACAAUCAGGUUUCUAAAUAAACAACUGGACCAUCAA-3'- Poly-A tail

Coding region

NCBI Matured mRNA sequence viewer

;

More about the NCBI sViewer.

DNA

DNA (Gene ID: 10956)

HGNC Gene symbol

OS9

HGNC Gene name

OS9 endoplasmic reticulum lectin

Chr. RefSeq ID

NC_000012.12

Chromosome N°

12 57694162.. 57720909

CDS location

57694162.. 57720909

DNA strand

strand +

CCDS ID

CCDS31843.1

Mouse ortholog gene

MGI:1924301

Gene Variant

ClinVar, dbVar

DNA Ensembl ID

ENSG00000135506

HGNC other symbol

OS-9, ERLEC2

UCSC ID

uc001spj.4

GeneBank

INSDC: AB002806

GeneCards

16994

MIM

609677

VEGA ID

OTTHUMG00000170284

Other links

ENA, DDBJ, Monarch

NCBI CDS Gene viewer

More about the NCBI sViewer.

NCBI CDS gene sequence (2004 bp)

5'-ATGGCGGCGGAAACGCTGCTGTCCAGTTTGTTAGGACTGCTGCTTCTGGGACTCCTGTTACCCGCAAGTCTGACCGGCGGTGTCGGGAGCCTGAACCTGGAGGAGCTGAGTGAGATGCGTTATGGGATCGAGATCCTGCCGTTGCCTGTCATGGGAGGGCAGAGCCAATCTTCGGACGTGGTGATTGTCTCCTCTAAGTACAAACAGCGCTATGAGTGTCGCCTGCCAGCTGGAGCTATTCACTTCCAGCGTGAAAGGGAGGAGGAAACACCTGCTTACCAAGGGCCTGGGATCCCTGAGTTGTTGAGCCCAATGAGAGATGCTCCCTGCTTGCTGAAGACAAAGGACTGGTGGACATATGAATTCTGTTATGGACGCCACATCCAGCAATACCACATGGAAGATTCAGAGATCAAAGGTGAAGTCCTCTATCTCGGCTACTACCAATCAGCCTTCGACTGGGATGATGAAACAGCCAAGGCCTCCAAGCAGCATCGTCTTAAACGCTACCACAGCCAGACCTATGGCAATGGGTCCAAGTGCGACCTTAATGGGAGGCCCCGGGAGGCCGAGGTTCGGTTCCTCTGTGACGAGGGTGCAGGTATCTCTGGGGACTACATCGATCGCGTGGACGAGCCCTTGTCCTGCTCTTATGTGCTGACCATTCGCACTCCTCGGCTCTGCCCCCACCCTCTCCTCCGGCCCCCACCCAGTGCTGCACCGCAGGCCATCCTCTGTCACCCTTCCCTACAGCCTGAGGAGTACATGGCCTACGTTCAGAGGCAAGCCGACTCAAAGCAGTATGGAGATAAAATCATAGAGGAGCTGCAAGATCTAGGCCCCCAAGTGTGGAGTGAGACCAAGTCTGGGGTGGCACCCCAAAAGATGGCAGGTGCGAGCCCGACCAAGGATGACAGTAAGGACTCAGATTTCTGGAAGATGCTTAATGAGCCAGAGGACCAGGCCCCAGGAGGGGAGGAGGTGCCGGCTGAGGAGCAGGACCCAAGCCCTGAGGCAGCAGATTCAGCTTCTGGTGCTCCCAATGATTTTCAGAACAACGTGCAGGTCAAAGTCATTCGAAGCCCTGCGGATTTGATTCGATTCATAGAGGAGCTGAAAGGTGGAACAAAAAAGGGGAAGCCAAATATAGGCCAAGAGCAGCCTGTGGATGATGCTGCAGAAGTCCCTCAGAGGGAACCAGAGAAGGAAAGGGGTGATCCAGAACGGCAGAGAGAGATGGAAGAAGAGGAGGATGAGGATGAGGATGAGGATGAAGATGAGGATGAACGGCAGTTACTGGGAGAATTTGAGAAGGAACTGGAAGGGATCCTGCTTCCGTCAGACCGAGACCGGCTCCGTTCGGAGGTGAAGGCTGGCATGGAGCGGGAACTGGAAAACATCATCCAGGAGACAGAGAAAGAGCTGGACCCAGATGGGCTGAAGAAGGAGTCAGAGCGGGATCGGGCAATGCTGGCTCTCACATCCACTCTCAACAAACTCATCAAAAGACTGGAGGAAAAACAGAGTCCAGAGCTGGTGAAGAAGCACAAGAAAAAGAGGGTTGTCCCCAAAAAGCCTCCCCCATCACCCCAACCTACAGAGGAGGATCCTGAGCACAGAGTCCGGGTCCGGGTCACCAAGCTCCGTCTCGGAGGCCCTAATCAGGATCTGACTGTCCTCGAGATGAAACGGGAAAACCCACAGCTGAAACAAATCGAGGGGCTGGTGAAGGAGCTGCTGGAGAGGGAGGGACTCACAGCTGCAGGGAAAATTGAGATCAAAATTGTCCGCCCATGGGCTGAAGGGACTGAAGAGGGTGCACGTTGGCTGACTGATGAGGACACGAGAAACCTCAAGGAGATCTTCTTCAATATCTTGGTGCCGGGAGCTGAAGAGGCCCAGAAGGAACGCCAGCGGCAGAAAGAGCTGGAGAGCAATTACCGCCGGGTGTGGGGCTCTCCAGGTGGGGAGGGCACAGGGGACCTGGACGAATTTGACTTCTGA-3'

NCBI CDS gene sequence with introns (location: 57694162.. 57720909) (26748 bp)Download

NCBI Gene Viewer (CDS + introns)

NCBI CDS gene sequence with introns, 5'UTR and 3'UTR (location: 57694132.. 57721557) (27426 bp)Download

NCBI Gene Viewer (CDS + introns + Untranslated regions)

NCBI gene sequence (location: [57694132 - 1000].. 57721557) (28426 bp)Download

NCBI Gene Viewer (CDS + introns + Untranslated regions + 1000 dNTP to potentially reach the promotor)