NCBI Summary
Calmegin is a testis-specific endoplasmic reticulum chaperone protein. CLGN may play a role in spermatogeneisis and infertility. [provided by RefSeq, Jul 2008].
Protein
Protein (NP_004353)
Calmegin
Calmegin
CLGN
calmegin
Undefined
Curated
calnexin-calreticulin like
CalnexinFamily
b-sandwich / ConA-like
Glc(a1-3)Man(a1-2)Man(a1-2)Man / glucosylated N-glycan
NP_004353
1047
O14967
ENSP00000326699.5
O14967
0.544
10.1016/j.bbagen.2014.04.012
neXtProt, PFAM, InterPro
Protein sequence and protein families (fasta) (610 amino acids) Download
MHFQAFWLCLGLLFISINAEFMDDDVETEDFEENSEEIDVNESELSSEIKYKTPQPIGEVYFAETFDSGRLAGWVLSKAKKDDMDEEISIYDGRWEIEELKENQVPGDRGLVLKSRAKHHAISAVLAKPFIFADKPLIVQYEVNFQDGIDCGGAYIKLLADTDDLILENFYDKTSYIIMFGPDKCGEDYKLHFIFRHKHPKTGVFEEKHAKPPDVDLKKFFTDRKTHLYTLVMNPDDTFEVLVDQTVVNKGSLLEDVVPPIKPPKEIEDPNDKKPEEWDERAKIPDPSAVKPEDWDESEPAQIEDSSVVKPAGWLDDEPKFIPDPNAEKPDDWNEDTDGEWEAPQILNPACRIGCGEWKPPMIDNPKYKGVWRPPLVDNPNYQGIWSPRKIPNPDYFEDDHPFLLTSFSALGLELWSMTSDIYFDNFIICSEKEVADHWAADGWRWKIMIANANKPGVLKQLMAAAEGHPWLWLIYLVTAGVPIALITSFCWPRKVKKKHKDTEYKKTDICIPQTKGVLEQEEKEEKAALEKPMDLEEEKKQNDGEMLEKEEESEPEEKSEEEIEIIEGQEESNQSNKSGSEDEMKEADESTGSGDGPIKSVRKRRVRKD
No structure currently available in the PDB RCSB Databank.
Structural models
AlphaFold v2: AF-O14967-F1Download
  Model Confidence:
  •    Very high (pLDDT > 90)
  •    Confident (90 > pLDDT > 70)
  •    Low (70 > pLDDT > 50)
  •    Very low (pLDDT < 50)

  AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

SWISS-MODEL structural models
SWISS-MODEL structural model
Modeller structural model (Homology modelling pipeline), Error: [0.85, 1.12] ÅDownload
The location of the lectin domain structural model is: 59-446
We infer [0.85, 1.12] Å as the interval of error of this structural model.
Template 1: 1JHN chain: A, P24643, NP_001003232.1, sequence identity: 70.6%, coverage: 100.0%, location in sequence: 61-458, (61-458 in PDB).
Template 2: 3O0W chain: A, P14211, NP_031617.1, sequence identity: 36.3%, coverage: 84.3%, location in sequence: 18-362, (18-362 in PDB).
Template 3: 6ENY chain: G, P27797, NP_004334.1, sequence identity: 36.3%, coverage: 84.3%, location in sequence: 18-386, (18-386 in PDB).
Show the alignment used for the construction of the structural model, Download.
Show the plot of DOPE energy score, Download.
Ligand
Glycan ligands from structural data
No crystal structures of complexes with glycan ligand.
Expression
Human Protein Atlas
GeneCards
mRNA expression in normal human tissues for CLGN Gene
ProteomicsDB
References
NCBI References (10 PubMed Identifiers)
  • Genome-Wide Gene-Sodium Interaction Analyses on Blood Pressure: The Genetic Epidemiology Network of Salt-Sensitivity Study. [27271309]
  • An organelle-specific protein landscape identifies novel diseases and molecular mechanisms. [27173435]
  • A Dynamic Protein Interaction Landscape of the Human Centrosome-Cilium Interface. [26638075]
  • Glycan specificity of a testis-specific lectin chaperone calmegin and effects of hydrophobic interactions. [24769397]
  • Genome-wide association with select biomarker traits in the Framingham Heart Study. [17903293]
  • Calmegin is required for fertilin alpha/beta heterodimerization and sperm fertility. [11784061]
  • Molecular chaperone calmegin localization to the endoplasmic reticulum of meiotic and post-meiotic germ cells in the mouse testis. [10495883]
  • Cloning and characterization of the human Calmegin gene encoding putative testis-specific chaperone. [9434179]
  • The putative chaperone calmegin is required for sperm fertility. [9177349]
  • Molecular cloning of a novel Ca(2+)-binding protein (calmegin) specifically expressed during male meiotic germ cell development. [8126001]
UniProt Main References (5 PubMed Identifiers)
  • Complete sequencing and characterization of 21,243 full-length human cDNAs. [14702039]
  • The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). [15489334]
  • Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. [17081983]
  • A developmentally regulated chaperone complex for the endoplasmic reticulum of male haploid germ cells. [17507649]
  • Initial characterization of the human central proteome. [21269460]
All isoforms of this gene containing a lectin domain
NP_001124147.1, NP_004353.1
RNA
RNA (Transcript ID: NM_004362.3)
NM_004362.3
calmegin, transcript variant 1
All isoforms of CLGN containing a lectin domain
ENST00000325617.10
122..1954 (or 140389224.. 140413078)
140388453.. 140427648
NCBI Matured mRNA sequence 2725 NTPDownload 
m7G-5')ppp(5'-AGUAGUCGCUGCUGCGCGGCCGCCGGCGGGACUGGUCUGAAGAGACGCGGGGACCAAGUGGCAACGACUUGGACAUCUGAGCUGUCACUGCCGAAAACAGGCCGCAAGAGAGAUAAUCAAUAUGCAUUUCCAAGCCUUUUGGCUAUGUUUGGGUCUUCUGUUCAUCUCAAUUAAUGCAGAAUUUAUGGAUGAUGAUGUUGAGACGGAAGACUUUGAAGAAAAUUCAGAAGAAAUUGAUGUUAAUGAAAGUGAACUUUCCUCAGAGAUUAAAUAUAAGACACCUCAACCUAUAGGAGAAGUAUAUUUUGCAGAAACUUUUGAUAGUGGAAGGUUGGCUGGAUGGGUCUUAUCAAAAGCAAAGAAAGAUGACAUGGAUGAGGAAAUUUCAAUAUACGAUGGAAGAUGGGAAAUUGAAGAGUUGAAAGAAAACCAGGUACCUGGUGACAGAGGACUGGUAUUAAAAUCUAGAGCAAAGCAUCAUGCAAUAUCUGCUGUAUUAGCAAAACCAUUCAUUUUUGCUGAUAAACCCUUGAUAGUUCAAUAUGAAGUAAAUUUUCAAGAUGGUAUUGAUUGUGGAGGUGCAUACAUUAAACUCCUAGCAGACACUGAUGAUUUGAUUCUGGAAAACUUUUAUGAUAAAACAUCCUAUAUCAUUAUGUUUGGACCAGAUAAAUGUGGAGAAGAUUAUAAACUUCAUUUUAUCUUCAGACAUAAACAUCCCAAAACUGGAGUUUUCGAAGAGAAACAUGCCAAACCUCCAGAUGUAGACCUUAAAAAGUUCUUUACAGACAGGAAGACUCAUCUUUAUACCCUUGUGAUGAAUCCAGAUGACACAUUUGAGGUGUUAGUUGAUCAAACAGUUGUAAACAAAGGAAGCCUCCUAGAGGAUGUGGUUCCUCCUAUCAAACCUCCCAAAGAAAUUGAAGAUCCCAAUGAUAAAAAACCUGAGGAAUGGGAUGAAAGAGCAAAAAUUCCUGAUCCUUCUGCCGUCAAACCAGAAGACUGGGAUGAAAGUGAACCUGCCCAAAUAGAAGAUUCAAGUGUUGUUAAACCUGCUGGCUGGCUUGAUGAUGAACCAAAAUUUAUCCCUGAUCCUAAUGCUGAAAAACCUGAUGACUGGAAUGAAGACACGGAUGGAGAAUGGGAGGCACCUCAGAUUCUUAAUCCAGCAUGUCGGAUUGGGUGUGGUGAGUGGAAACCUCCCAUGAUAGAUAACCCAAAAUACAAAGGAGUAUGGAGACCUCCACUGGUCGAUAAUCCUAACUAUCAGGGAAUCUGGAGUCCUCGAAAAAUUCCUAAUCCAGAUUAUUUCGAAGAUGAUCAUCCAUUUCUUCUGACUUCUUUCAGUGCUCUUGGUUUAGAGCUUUGGUCUAUGACCUCUGAUAUCUACUUUGAUAAUUUUAUUAUCUGUUCGGAAAAGGAAGUAGCAGAUCACUGGGCUGCAGAUGGUUGGAGAUGGAAAAUAAUGAUAGCAAAUGCUAAUAAGCCUGGUGUAUUAAAACAGUUAAUGGCAGCUGCUGAAGGGCACCCAUGGCUUUGGUUGAUUUAUCUUGUGACAGCAGGAGUGCCAAUAGCAUUAAUUACUUCAUUUUGUUGGCCAAGAAAAGUAAAGAAAAAACAUAAAGAUACAGAGUAUAAAAAAACCGACAUAUGUAUACCACAAACAAAAGGAGUACUAGAGCAAGAAGAAAAGGAAGAGAAAGCAGCCCUGGAAAAACCAAUGGACCUGGAAGAGGAAAAAAAGCAAAAUGAUGGUGAAAUGCUUGAAAAAGAAGAGGAAAGUGAACCUGAGGAAAAGAGUGAAGAAGAAAUUGAAAUCAUAGAAGGGCAAGAAGAAAGUAAUCAAUCAAAUAAGUCUGGGUCAGAGGAUGAGAUGAAAGAAGCAGAUGAGAGCACAGGAUCUGGAGAUGGGCCGAUAAAGUCAGUACGCAAAAGAAGAGUACGAAAGGACUAAACUAGAUUGAAAUAUUUUUAAUUCCCGAGAGGGAUGUUUGGCAUUGUAAAAAUCAGCAUGCCAGACCUGAACUUUAAUCAGUCUGCACAUCCUGUUUCUAAUAUCUAGCAACAUUAUAUUCUUUCAGACAUUUAUUUUAGUCCUUCAUUUCAGAGGAAAAAGAAGCAACUUUGAAGUUACCUCAUCUUUGAAUUUAGAAUAAAAGUGGCACAUUACAUAUCGGAUCUAAGAGAUUAAUACCAUUAGAAGUUACACAGUUUUAGUUGUUUGGAGAUAGUUUUGGUUUGUACAGAACAAAAUAAUAUGUAGCAGCUUCAUUGCUAUUGGAAAAAUCAGUUAUUGGAAUUUCCACUUAAAUGGCUAUACAACAAUAUAACUGGUAGUUCUAUAAUAAAAAUGAGCAUAUGUUCUGUUGUGAAGAGCUAAAUGCAAUAAAGUUUCUGUAUGGUUGUUUGAUUCUAUCAACAAUUGAAAGUGUUGUAUAUGACCCACAUUUACCUAGUUUGUGUCAAAUUAUAGUUACAGUGAGUUGUUUGCUUAAAUUAUAGAUUCCUUUAAGGACAUGCCUUGUUCAUAAAAUCACUGGAUUAUAUUGCAGCAUAUUUUACAUUUGAAUACAAGGAUAAUGGGUUUUAUCAAAACAAAAUGAUGUACAGAUUUUUUUUCAAGUUUUUAUAGUUGCUUUAUGCCAGAGUGGUUUACCCCAUUCACAAAAUUUCUUAUGCAUACAUUGCUAUUGAAAAUAAAAUUUAAAUAUUUUUUCAUCCUGAA-3'- Poly-A tail
  • Coding region
NCBI Matured mRNA sequence viewer
;
More about the NCBI sViewer.
DNA
DNA (Gene ID: 1047)
CLGN
calmegin
NC_000004.12
4  140389224.. 140413078
140389224.. 140413078
strand -
CCDS3751.1
MGI:107472
ClinVar, dbVar
ENSG00000153132
uc003iii.4
INSDC: D86322
2060
601858
OTTHUMG00000133414
ENA, DDBJ, Monarch
NCBI CDS Gene viewer
More about the NCBI sViewer.
NCBI CDS gene sequence (1833 bp)
5'-ATGCATTTCCAAGCCTTTTGGCTATGTTTGGGTCTTCTGTTCATCTCAATTAATGCAGAATTTATGGATGATGATGTTGAGACGGAAGACTTTGAAGAAAATTCAGAAGAAATTGATGTTAATGAAAGTGAACTTTCCTCAGAGATTAAATATAAGACACCTCAACCTATAGGAGAAGTATATTTTGCAGAAACTTTTGATAGTGGAAGGTTGGCTGGATGGGTCTTATCAAAAGCAAAGAAAGATGACATGGATGAGGAAATTTCAATATACGATGGAAGATGGGAAATTGAAGAGTTGAAAGAAAACCAGGTACCTGGTGACAGAGGACTGGTATTAAAATCTAGAGCAAAGCATCATGCAATATCTGCTGTATTAGCAAAACCATTCATTTTTGCTGATAAACCCTTGATAGTTCAATATGAAGTAAATTTTCAAGATGGTATTGATTGTGGAGGTGCATACATTAAACTCCTAGCAGACACTGATGATTTGATTCTGGAAAACTTTTATGATAAAACATCCTATATCATTATGTTTGGACCAGATAAATGTGGAGAAGATTATAAACTTCATTTTATCTTCAGACATAAACATCCCAAAACTGGAGTTTTCGAAGAGAAACATGCCAAACCTCCAGATGTAGACCTTAAAAAGTTCTTTACAGACAGGAAGACTCATCTTTATACCCTTGTGATGAATCCAGATGACACATTTGAGGTGTTAGTTGATCAAACAGTTGTAAACAAAGGAAGCCTCCTAGAGGATGTGGTTCCTCCTATCAAACCTCCCAAAGAAATTGAAGATCCCAATGATAAAAAACCTGAGGAATGGGATGAAAGAGCAAAAATTCCTGATCCTTCTGCCGTCAAACCAGAAGACTGGGATGAAAGTGAACCTGCCCAAATAGAAGATTCAAGTGTTGTTAAACCTGCTGGCTGGCTTGATGATGAACCAAAATTTATCCCTGATCCTAATGCTGAAAAACCTGATGACTGGAATGAAGACACGGATGGAGAATGGGAGGCACCTCAGATTCTTAATCCAGCATGTCGGATTGGGTGTGGTGAGTGGAAACCTCCCATGATAGATAACCCAAAATACAAAGGAGTATGGAGACCTCCACTGGTCGATAATCCTAACTATCAGGGAATCTGGAGTCCTCGAAAAATTCCTAATCCAGATTATTTCGAAGATGATCATCCATTTCTTCTGACTTCTTTCAGTGCTCTTGGTTTAGAGCTTTGGTCTATGACCTCTGATATCTACTTTGATAATTTTATTATCTGTTCGGAAAAGGAAGTAGCAGATCACTGGGCTGCAGATGGTTGGAGATGGAAAATAATGATAGCAAATGCTAATAAGCCTGGTGTATTAAAACAGTTAATGGCAGCTGCTGAAGGGCACCCATGGCTTTGGTTGATTTATCTTGTGACAGCAGGAGTGCCAATAGCATTAATTACTTCATTTTGTTGGCCAAGAAAAGTAAAGAAAAAACATAAAGATACAGAGTATAAAAAAACCGACATATGTATACCACAAACAAAAGGAGTACTAGAGCAAGAAGAAAAGGAAGAGAAAGCAGCCCTGGAAAAACCAATGGACCTGGAAGAGGAAAAAAAGCAAAATGATGGTGAAATGCTTGAAAAAGAAGAGGAAAGTGAACCTGAGGAAAAGAGTGAAGAAGAAATTGAAATCATAGAAGGGCAAGAAGAAAGTAATCAATCAAATAAGTCTGGGTCAGAGGATGAGATGAAAGAAGCAGATGAGAGCACAGGATCTGGAGATGGGCCGATAAAGTCAGTACGCAAAAGAAGAGTACGAAAGGACTAA-3'
NCBI CDS gene sequence with introns (location: 140389224.. 140413078) (23855 bp)Download
NCBI Gene Viewer (CDS + introns)
NCBI CDS gene sequence with introns, 5'UTR and 3'UTR (location: 140388453.. 140427648) (39196 bp)Download
NCBI Gene Viewer (CDS + introns + Untranslated regions)
NCBI gene sequence (location: [140388453.. 140427648 + 1000]) (40196 bp)Download
NCBI Gene Viewer (CDS + introns + Untranslated regions + 1000 dNTP to potentially reach the promotor)
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