SMTL ID : 3k41.1

Crystal structure of sCD-MPR mutant E19Q/K137M bound to Man-6-P

Coordinates

PDB Format

Method

X-RAY DIFFRACTION 1.90 Å

Oligo State

homo-dimer

Ligands

1 x NAG- NAG- BMA- MAN: alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose(Post Translational Modification)

NAG-NAG-BMA-MAN.1: 4 residues within 4Å: Chain A: S.60, R.79, N.81, E.82 No protein-ligand interaction detected (PLIP)

1 x NAG- NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose(Non-covalent)

NAG-NAG.2: 4 residues within 4Å: Chain B: S.60, R.79, N.81, E.82 No protein-ligand interaction detected (PLIP)

2 x M6D: 6-O-phosphono-beta-D-mannopyranose(Non-functional Binders)

M6D.3: 11 residues within 4Å: Chain A: Y.45, Q.66, K.73, Y.102, D.103, N.104, H.105, R.111, E.133, R.135, Y.143 20 PLIP interactions: 20 interactions with chain A Hydrogen bonds: A:Y.45, A:Y.45, A:Q.66, A:Q.66, A:D.103, A:N.104, A:N.104, A:H.105, A:R.111, A:E.133, A:R.135, A:Y.143 Water bridges: A:Y.47, A:K.73, A:K.73, A:D.103, A:D.103 Salt bridges: A:K.73, A:H.105, A:R.111 M6D.4: 10 residues within 4Å: Chain B: Y.45, Q.66, Y.102, D.103, N.104, H.105, R.111, E.133, R.135, Y.143 12 PLIP interactions: 12 interactions with chain B Hydrogen bonds: B:Q.66, B:Q.66, B:D.103, B:N.104, B:N.104, B:H.105, B:R.111, B:E.133, B:R.135, B:Y.143 Salt bridges: B:H.105, B:R.111

Links

RCSB   PDBe   PDBj   PDBsum   CATH   PLIP

Citation

Olson, L.J. et al., Intermonomer interactions are essential for lysosomal enzyme binding by the cation-dependent mannose 6-phosphate receptor. Biochemistry (2010)

Release Date

2009-11-24

Peptides

Cation-dependent mannose-6-phosphate receptor: AB

SMTL:PDB

SMTL Chain Id:
PDB Chain Id:

A
A

B
B

Cation-dependent mannose-6-phosphate receptor