SMTL ID : 2xjv.1

X-ray structure of the N-terminal domain of the flocculin Flo5 from Saccharomyces cerevisiae with mutation D201T in complex with calcium and glucose

Coordinates

PDB Format

Method

X-RAY DIFFRACTION 1.74 Å

Oligo State

monomer

Ligands

2 x CA: CALCIUM ION(Non-covalent)

CA.1: 6 residues within 4Å: Chain A: D.147, D.148, N.211, V.213, W.215 Ligands: BGC.6 4 PLIP interactions: 4 interactions with chain A Metal complexes: A:D.147, A:D.148, A:V.213, A:W.215 CA.2: 2 residues within 4Å: Chain A: S.157, E.161 5 PLIP interactions: 2 interactions with chain A, 3 Ligand-Water interactions Metal complexes: A:S.157, A:E.161, H2O.8, H2O.8, H2O.8

2 x NA: SODIUM ION(Non-functional Binders)

NA.3: 3 residues within 4Å: Chain A: P.73, F.81, P.82 No protein-ligand interaction detected (PLIP) NA.5: 3 residues within 4Å: Chain A: D.69, N.71, Q.85 3 PLIP interactions: 3 interactions with chain A Hydrogen bonds: A:D.69, A:N.71, A:Q.85

1 x CL: CHLORIDE ION(Non-functional Binders)

CL.4: 6 residues within 4Å: Chain A: Y.89, C.94, K.95, G.96, M.97, G.98 Ligand excluded by PLIP

2 x BGC: beta-D-glucopyranose(Non-covalent)

BGC.6: 7 residues within 4Å: Chain A: D.147, D.148, K.181, V.213, S.214, W.215 Ligands: CA.1 6 PLIP interactions: 6 interactions with chain A Hydrogen bonds: A:K.181, A:K.181, A:V.213, A:S.214, A:S.214, A:W.215 BGC.7: 7 residues within 4Å: Chain A: D.65, I.66, S.67, D.112, L.113, F.114, G.115 7 PLIP interactions: 7 interactions with chain A Hydrogen bonds: A:F.114, A:G.115 Water bridges: A:D.65, A:S.67, A:S.67, A:D.69, A:F.114

Links

RCSB   PDBe   PDBj   PDBsum   CATH   PLIP

Citation

Veelders, M. et al., Structural Basis of Flocculin-Mediated Social Behavior in Yeast. Proc.Natl.Acad.Sci.USA (2010)

Release Date

2010-12-15

Peptides

FLOCCULATION PROTEIN FLO5: A

SMTL:PDB

SMTL Chain Id:
PDB Chain Id:

A
A

FLOCCULATION PROTEIN FLO5