SMTL ID : 1w3f.1

Crystal structure of the hemolytic lectin from the mushroom Laetiporus sulphureus complexed with N-acetyllactosamine in the gamma motif

Coordinates

PDB Format

Method

X-RAY DIFFRACTION 2.58 Å

Oligo State

homo-hexamer

Ligands

6 x NDG- GAL: beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-alpha-D-glucopyranose(Non-functional Binders)

NDG-GAL.1: 12 residues within 4Å: Chain A: Y.81, V.121, R.123, H.125, L.126, P.128, W.131, H.133, F.139, D.141, Q.142 Chain B: P.38 3 PLIP interactions: 3 interactions with chain A Hydrogen bonds: A:R.123, A:H.125, A:Q.142, A:R.123, A:R.123 Water bridges: A:H.133, A:Q.127 NDG-GAL.7: 12 residues within 4Å: Chain B: Y.81, V.121, R.123, H.125, L.126, P.128, W.131, H.133, F.139, D.141, Q.142 Chain C: P.38 3 PLIP interactions: 3 interactions with chain B Hydrogen bonds: B:R.123, B:H.125, B:Q.142, B:R.123, B:R.123 Water bridges: B:H.133, B:Q.127 NDG-GAL.13: 12 residues within 4Å: Chain A: P.38 Chain C: Y.81, V.121, R.123, H.125, L.126, P.128, W.131, H.133, F.139, D.141, Q.142 3 PLIP interactions: 3 interactions with chain C Hydrogen bonds: C:R.123, C:H.125, C:Q.142, C:R.123, C:R.123 Water bridges: C:H.133, C:Q.127 NDG-GAL.19: 12 residues within 4Å: Chain D: Y.81, V.121, R.123, H.125, L.126, P.128, W.131, H.133, F.139, D.141, Q.142 Chain E: P.38 3 PLIP interactions: 3 interactions with chain D Hydrogen bonds: D:R.123, D:H.125, D:Q.142, D:R.123, D:R.123 Water bridges: D:Q.127 NDG-GAL.25: 12 residues within 4Å: Chain E: Y.81, V.121, R.123, H.125, L.126, P.128, W.131, H.133, F.139, D.141, Q.142 Chain F: P.38 3 PLIP interactions: 3 interactions with chain E Hydrogen bonds: E:R.123, E:H.125, E:Q.142, E:R.123, E:R.123 Water bridges: E:Q.127 NDG-GAL.31: 12 residues within 4Å: Chain D: P.38 Chain F: Y.81, V.121, R.123, H.125, L.126, P.128, W.131, H.133, F.139, D.141, Q.142 3 PLIP interactions: 3 interactions with chain F Hydrogen bonds: F:R.123, F:H.125, F:Q.142, F:R.123, F:R.123 Water bridges: F:Q.127

30 x GOL: GLYCEROL(Non-functional Binders)(Non-covalent)

GOL.2: 6 residues within 4Å: Chain A: F.25, R.27, S.37, D.45, Q.46 Chain C: F.139 Ligand excluded by PLIP GOL.3: 7 residues within 4Å: Chain A: D.150, M.151, S.152, I.153, T.214, A.215, K.286 Ligand excluded by PLIP GOL.4: 6 residues within 4Å: Chain A: F.73, R.75, I.85, Y.91, D.93, N.94 Ligand excluded by PLIP GOL.5: 5 residues within 4Å: Chain A: T.245, F.246, S.247 Chain C: T.205, I.297 Ligand excluded by PLIP GOL.6: 5 residues within 4Å: Chain A: D.41, Q.42, T.44, Y.47, K.66 Ligand excluded by PLIP GOL.8: 6 residues within 4Å: Chain A: F.139 Chain B: F.25, R.27, S.37, D.45, Q.46 Ligand excluded by PLIP GOL.9: 7 residues within 4Å: Chain B: D.150, M.151, S.152, I.153, T.214, A.215, K.286 Ligand excluded by PLIP GOL.10: 6 residues within 4Å: Chain B: F.73, R.75, I.85, Y.91, D.93, N.94 Ligand excluded by PLIP GOL.11: 5 residues within 4Å: Chain A: T.205, I.297 Chain B: T.245, F.246, S.247 Ligand excluded by PLIP GOL.12: 5 residues within 4Å: Chain B: D.41, Q.42, T.44, Y.47, K.66 Ligand excluded by PLIP GOL.14: 6 residues within 4Å: Chain B: F.139 Chain C: F.25, R.27, S.37, D.45, Q.46 Ligand excluded by PLIP GOL.15: 7 residues within 4Å: Chain C: D.150, M.151, S.152, I.153, T.214, A.215, K.286 Ligand excluded by PLIP GOL.16: 6 residues within 4Å: Chain C: F.73, R.75, I.85, Y.91, D.93, N.94 Ligand excluded by PLIP GOL.17: 5 residues within 4Å: Chain B: T.205, I.297 Chain C: T.245, F.246, S.247 Ligand excluded by PLIP GOL.18: 5 residues within 4Å: Chain C: D.41, Q.42, T.44, Y.47, K.66 Ligand excluded by PLIP GOL.20: 6 residues within 4Å: Chain D: F.25, R.27, S.37, D.45, Q.46 Chain F: F.139 Ligand excluded by PLIP GOL.21: 7 residues within 4Å: Chain D: D.150, M.151, S.152, I.153, T.214, A.215, K.286 Ligand excluded by PLIP GOL.22: 6 residues within 4Å: Chain D: F.73, R.75, I.85, Y.91, D.93, N.94 Ligand excluded by PLIP GOL.23: 5 residues within 4Å: Chain D: T.245, F.246, S.247 Chain F: T.205, I.297 Ligand excluded by PLIP GOL.24: 5 residues within 4Å: Chain D: D.41, Q.42, T.44, Y.47, K.66 Ligand excluded by PLIP GOL.26: 6 residues within 4Å: Chain D: F.139 Chain E: F.25, R.27, S.37, D.45, Q.46 Ligand excluded by PLIP GOL.27: 7 residues within 4Å: Chain E: D.150, M.151, S.152, I.153, T.214, A.215, K.286 Ligand excluded by PLIP GOL.28: 6 residues within 4Å: Chain E: F.73, R.75, I.85, Y.91, D.93, N.94 Ligand excluded by PLIP GOL.29: 5 residues within 4Å: Chain D: T.205, I.297 Chain E: T.245, F.246, S.247 Ligand excluded by PLIP GOL.30: 5 residues within 4Å: Chain E: D.41, Q.42, T.44, Y.47, K.66 Ligand excluded by PLIP GOL.32: 6 residues within 4Å: Chain E: F.139 Chain F: F.25, R.27, S.37, D.45, Q.46 Ligand excluded by PLIP GOL.33: 7 residues within 4Å: Chain F: D.150, M.151, S.152, I.153, T.214, A.215, K.286 Ligand excluded by PLIP GOL.34: 6 residues within 4Å: Chain F: F.73, R.75, I.85, Y.91, D.93, N.94 Ligand excluded by PLIP GOL.35: 5 residues within 4Å: Chain E: T.205, I.297 Chain F: T.245, F.246, S.247 Ligand excluded by PLIP GOL.36: 5 residues within 4Å: Chain F: D.41, Q.42, T.44, Y.47, K.66 Ligand excluded by PLIP

Links

RCSB   PDBe   PDBj   PDBsum   CATH   PLIP

Citation

Mancheno, J.M. et al., Structural Analysis of the Laetiporus Sulphureus Hemolytic Pore-Forming Lectin in Complex with Sugars. J.Biol.Chem. (2005)

Release Date

2005-02-01

Peptides

HEMOLYTIC LECTIN FROM LAETIPORUS SULPHUREUS: ABCDEF

SMTL:PDB

SMTL Chain Id:
PDB Chain Id:

A
A

B
A

C
A

D
A

E
A

F
A

HEMOLYTIC LECTIN FROM LAETIPORUS SULPHUREUS

Related Entries With Identical Sequence