SMTL ID : 1qmo.1

Structure of FRIL, a legume lectin that delays hematopoietic progenitor maturation

Coordinates

PDB Format

Method

X-RAY DIFFRACTION 3.50 Å

Oligo State

hetero-4-4-mer

Ligands

4 x CA: CALCIUM ION(Non-covalent)

CA.1: 5 residues within 4Å: Chain A: G.104 Chain E: D.9, Y.11, N.13, D.18 4 PLIP interactions: 4 interactions with chain E Metal complexes: E:D.9, E:D.9, E:Y.11, E:D.18 CA.4: 5 residues within 4Å: Chain B: G.104 Chain F: D.9, Y.11, N.13, D.18 4 PLIP interactions: 4 interactions with chain F Metal complexes: F:D.9, F:D.9, F:Y.11, F:D.18 CA.7: 5 residues within 4Å: Chain C: G.104 Chain G: D.9, Y.11, N.13, D.18 4 PLIP interactions: 4 interactions with chain G Metal complexes: G:D.9, G:D.9, G:Y.11, G:D.18 CA.10: 5 residues within 4Å: Chain D: G.104 Chain H: D.9, Y.11, N.13, D.18 4 PLIP interactions: 4 interactions with chain H Metal complexes: H:D.9, H:D.9, H:Y.11, H:D.18

4 x MN: MANGANESE (II) ION(Non-covalent)

MN.2: 5 residues within 4Å: Chain E: E.7, D.9, D.18, H.23, S.33 4 PLIP interactions: 4 interactions with chain E Metal complexes: E:E.7, E:D.9, E:D.18, E:H.23 MN.5: 5 residues within 4Å: Chain F: E.7, D.9, D.18, H.23, S.33 4 PLIP interactions: 4 interactions with chain F Metal complexes: F:E.7, F:D.9, F:D.18, F:H.23 MN.8: 5 residues within 4Å: Chain G: E.7, D.9, D.18, H.23, S.33 4 PLIP interactions: 4 interactions with chain G Metal complexes: G:E.7, G:D.9, G:D.18, G:H.23 MN.11: 5 residues within 4Å: Chain H: E.7, D.9, D.18, H.23, S.33 4 PLIP interactions: 4 interactions with chain H Metal complexes: H:E.7, H:D.9, H:D.18, H:H.23

4 x MAN: alpha-D-mannopyranose(Non-covalent)

MAN.3: 10 residues within 4Å: Chain A: A.85, D.86, G.103, G.104 Chain E: Y.11, N.13, Y.16, G.97, Q.98, D.99 7 PLIP interactions: 6 interactions with chain E, 1 interactions with chain A Hydrogen bonds: E:N.13, E:Y.16, E:G.97, E:Q.98, E:D.99, E:D.99, A:G.104 MAN.6: 10 residues within 4Å: Chain B: A.85, D.86, G.103, G.104 Chain F: Y.11, N.13, Y.16, G.97, Q.98, D.99 8 PLIP interactions: 7 interactions with chain F, 1 interactions with chain B Hydrogen bonds: F:N.13, F:Y.16, F:Y.16, F:G.97, F:Q.98, F:D.99, F:D.99, B:G.104 MAN.9: 10 residues within 4Å: Chain C: A.85, D.86, G.103, G.104 Chain G: Y.11, N.13, Y.16, G.97, Q.98, D.99 8 PLIP interactions: 7 interactions with chain G, 1 interactions with chain C Hydrogen bonds: G:N.13, G:Y.16, G:Y.16, G:G.97, G:Q.98, G:D.99, G:D.99, C:G.104 MAN.12: 10 residues within 4Å: Chain D: A.85, D.86, G.103, G.104 Chain H: Y.11, N.13, Y.16, G.97, Q.98, D.99 7 PLIP interactions: 6 interactions with chain H, 1 interactions with chain D Hydrogen bonds: H:N.13, H:Y.16, H:G.97, H:Q.98, H:D.99, H:D.99, D:G.104

Links

RCSB   PDBe   PDBj   PDBsum   CATH   PLIP

Citation

Hamelryck, T.W. et al., The Role of Weak Protein-Protein Interactions in Multivalent Lectin-Carbohydrate Binding: Crystal Structure of Cross-Linked Fril. J.Mol.Biol. (2000)

Release Date

1999-10-10

Peptides

MANNOSE BINDING LECTIN, FRIL: ABCD
MANNOSE BINDING LECTIN, FRIL: EFGH

SMTL:PDB

SMTL Chain Id:
PDB Chain Id:

A
A

B
B

C
C

D
D

E
E

F
F

G
G

H
H

MANNOSE BINDING LECTIN, FRIL

MANNOSE BINDING LECTIN, FRIL